Abstract
Rabbits were immunized with human or bovine albumin at different intervals after birth and antibody formation was studied by haemagglutination of red cells sensitized with the relevant antigen. The intraperitoneal injection of antigen in amounts of 5 mg. induced antibody formation in some litters 16–20 days after immunization, if the animals were over three days old when immunized. In younger rabbits the same dose induced tolerance. Even when different methods of enhancing the effect of the antigen (Freund’s adjuvant, Al (OH)3, antigen-conjugated red cells, immune precipitates) or very small doses of antigen were used, antibody formation was still not detected before the 20th day of life. The use of131I-BSA did not demonstrate the immune phase of elimination of the antigen during 17 days after administration of the antigen, even in rabbits immunized 14 days after birth.
The relationship of antibody formation to the induction of tolerance and the difference in the response of newborn rabbits to immunization with the different types of antigen is discussed.
Abstract
Крольчат иммунизировали через различные сроки после рождения человеческим или говяжьим сывороточным альбумином и исследовали образование антител методом гемагглютинации эритроцитов, сенсибилизированных соответствующим антигеном. При внутрибрюшинном введении в количестве 5 мг антиген у некоторых пометов крольчат вызывал образование антител через 16–20 дней после иммунизации—при условии, что в момент иммунизации крольчатам было больше 3 дней. У более молодых животных эта доза вызывала развитие толерантности. Даже при применении различных способов потенцирования действия антигена (адъювант Фройнда, Al(OH)3, связывание антигена эритроцитами, иммунные преципитаты) и при введении весьма ограниченных доз антигена не удавалось отметить возникновения антител раньше 20-го дня жизни крольчат. При применении BSA-J131 не удавалось обнаружить иммунной фазы выделения антигена вплоть до 17-го дня после его введения—даже у крольчат, иммунизированных на 14-й день после рождения.—Обсуждаются отношение образования антител к созданию толерантности и различия в реакции новорожденных крольчат на иммунизацию разными типами антигенов.
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Říha, I. Antibody formation in young rabbits immunized with heterologous serum proteins. Folia Microbiol 8, 1–8 (1963). https://doi.org/10.1007/BF02868759
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DOI: https://doi.org/10.1007/BF02868759