Skip to main content
SpringerLink
Log in

Study on antitumor and immunomodulating activities of polysaccharide fractions fromPanax ginseng: Comparison of effects of neutral and acidic polysaccharide fraction

  • Original Articles
  • Published:
Archives of Pharmacal Research Aims and scope Submit manuscript

Abstract

The crude polysaccharide fromPanax ginseng prepared by hot water extraction and precipitation with ethanol was further fractionated into neutral and acidic fractions by DEAE-cellulose ion exchange chromatography. The chemical compositions were 85.0% carbohydrate and 15.0% protein for the neutral fraction, and 28.4% carbohydrate, 10.0% protein and 29.0% uronic acid for the acidic fraction. The acidic fraction was more effective in increasing of the ratio of spleen to body weight, the number of antibody secreting cells to SRBC and phagocytic activity of reticuloendothelial system, as well as antitumor activity against the solid form of sarcoma 180 in ICR mice than the neutral fraction. All polysaccharide fractions were mitogenic to cultured spleen cells of C57BL/6 mice. However, FA was different from FN in the co-mitogenicities with lectin mitogens. Both crude and acidic fractions potentiated remarkably the mitogenic activity of PHA-P or LPS in dose-dependent manner but neutral fraction enhanced only that of LPS. Three polysaccharide fractoins had no effect on that of Con A. These results suggest that the acidic fraction may stimulate B and T cells as well as macrophages while the neutral fraction may stimulate only B cells and macropages.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Ha, T. Y., Lee, J. H. and Kim, S. H.: Effect ofPanax ginseng on the graft-versus-host reaction, production of leucocyte migration inhibitory factor and expulsion of aldultTrichinealla Spiralis in mice.J. Korean Soc. Microbiol. 21, 133(1986).

    Google Scholar 

  2. Jie, Y. H., Cammisuli, S. and Baggiolini M.: Immunomodulatory effects ofPanax ginseng C. A. Meyer in the mouse.Agents and Actions 15, 386 (1984).

    Article  PubMed  CAS  Google Scholar 

  3. Singh, V. K., Agarwal, S. S. and Gupta, B. M.: Immunomodulatory activity ofPanax ginseng extract.Panta Medica 50, 462 (1984).

    Article  CAS  Google Scholar 

  4. Matsuda, H., Hasegawa, T. and Kubo, M.: Pharmacological study onPanax ginseng C. A. Meyer. VII. Protective effect of red ginseng on infection (1). On phagocytic activity of mouse reticuloendotherial system.Yakugaku Zasshi 105, 948 (1985).

    PubMed  CAS  Google Scholar 

  5. Kim, M. J. and Jung, N. P.: The effect of ginseng saponin on the mouse immune system.Korean J. Ginseng Sci. 11, 130 (1987).

    Google Scholar 

  6. Matsuda, H., Kubo, M., Tani, T., Kitaguwa, I. and Mizuno, M.: Pharmacological study onPanax ginseng C. A. Meyer (IX). Protective effect of red ginseng on infection (2) on phagocytic activity of mouse reticuloendotherial system.Shoyakugaku Zasshi 41, 131 (1987).

    Google Scholar 

  7. Park, H. W., Kim, S. C. and Jung, N. P.: The effect of ginseng saponin fractions on humoral immunity of mouse.Korean J. Ginseng Sic. 12, 63 (1988).

    Google Scholar 

  8. Kim, M. N. and Jung, N. P.: The effect of ginseng saponin fraction on NK activity in mice.Korean J. Ginseng Sci. 13, 223 (1989)

    Google Scholar 

  9. Moon, C. K., Sim, K. S., Lee, S. H., Park, K. S., Yun, Y. P., Ha, B. J. and Lee, C. C.: Antitumor activity of some phytobased polysaccharides and their effects on the immune function.Arch. Pharm. Res. 6, 123 (1983).

    Article  CAS  Google Scholar 

  10. Wang, B. X., Lhi, J. C. and Cui, A. J.: Study on anti-tumor activity of ginseng polysaccharides, 5th, Int. Ginseng Symp. (Abstract) (1988).

  11. Quian, B. C., Zang, X. X., Li, B., Xu, C. Y. and Peng, X. Y.: Effects of ginseng polysaccharides on tumor and immunological function in tumor-bearing mice.Acta. Pharmacol. Sin. 8, 277 (1987).

    Google Scholar 

  12. Yuan, X. N., Shang, X. H., Jin, M. L. and Shang, K.: Effects of ginseng root polysaccharides on cyclophoslphamide-suppressed immunity.Shenyang Yaoxueyuan Xeuebao 3, 162 (1986).

    CAS  Google Scholar 

  13. Konno, C., Sugiyama, K., Kano, M., Takahashi, M. and Hikino, H.: Isolation and hypoglycemic activity of panaxans A, B, C, D and E, glycans ofPanax ginseng roots.Planta Medica 50, 443 (1984).

    Article  Google Scholar 

  14. Hikino, H., Oshima, Y., Suzuki, Y. and Konno, C.: Isolation and hyploglycemic activity of panaxans F. G. H. I. J. K. and L, glycans ofPanax ginseng roots.Shoyakigaku Zasshi 39, 331 (1985).

    CAS  Google Scholar 

  15. Konno, C. and Hikino, H.: Isolation and hypoglycemic activity of panaxans M, N, O, and P, glycans ofPanax ginseng roots.Int. J. Crude Drug. Res. 25, 53 (1987).

    CAS  Google Scholar 

  16. Kanno, C., Murakami, M., Oshima, Y. and Hikino, H.: Isolation and hypoglycemic activity of panaxans Q, R, S, T and U, glycans ofPanax ginseng roots.J. Ethnopharmacol. 14, 69 (1985).

    Article  Google Scholar 

  17. Gao, Q. P., Kiyohara, H., Cyong, J. C. and Yamada, H.: Chemical properties and anti-complementary activities of polysaccharide fractions from roots and leaves ofPanax ginseng.Planta Medica 55, 9 (1989).

    Article  PubMed  CAS  Google Scholar 

  18. Eun, S. M., Hung, N. K., Nam, L. K. and Cheung, K. Y.: Growth promoting activity of a macromolecular fraction from fresh ginseng.Korean J. Ginseng Sci. 13, 215 (1989).

    CAS  Google Scholar 

  19. Lee, S. D. and Okuda, H.: Effect of acidic polysaccharide of Korean red ginseng on lipolytic action of toxohormone-L from cancerous ascites fluid.Korean J. Ginseng Sci. 14, 67 (1990).

    CAS  Google Scholar 

  20. Ohtani, K., Mizutani, K., Hatono, S., Kasai, R., Sumino, R., Shiota, T., Ushi, M., Fuwa, T. and Tanaka, O.: Sanchinan-A, a reticuloendothelial system activating arabinogalactan from Sanchi-Ginseng (Roots ofPanax notoginseng).Planta Medica 53, 166 (1987).

    Article  PubMed  CAS  Google Scholar 

  21. Dimler, R. J., Schaefer, W. C., Wise, C. S. and Rist, C. E.: Quantitative paper chromatography of D-glucose and its oligosaccharides.Anal. Chem. 24, 1411 (1952).

    Article  CAS  Google Scholar 

  22. Chaplin, M. F. and Kennedy, J. F.:Carbohydrate Analysis, 1st ed., IRL Press, Oxford, p. 5 (1987).

    Google Scholar 

  23. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J.: Protein measurment with the folin phenol reagent.J. Biol. Chem. 193, 265 (1951).

    PubMed  CAS  Google Scholar 

  24. Cunningham, A. J. and Szenberg, A.: Futher improvements in the plaque technique for detecting single antibody forming cells.Immunology 14, 599 (1968).

    PubMed  CAS  Google Scholar 

  25. Biozzi, G., Benacerraf, B., Stiffel, C., and Halpern, B. N.: Quantitative study of the granulopetic activity of the reticuloendotherial system.Br. J. Exp. Patho. 34, 447 (1954).

    Google Scholar 

  26. Chihara, G., Hamuro, J., Maeda, Y., Ayai, Y. and Fukuoka, F.: Fractionation and purification of the polysaccharides with marked anti-tumor activity, especially lentinan fromLentinus edodes (Berk.).Sing. Cancer Res. 30, 2776 (1970).

    CAS  Google Scholar 

  27. Komatsu, N., Okubo, S., Kikumoto, S., Kimura, K., Saito, G. and Sakai, S.: Host mediated anti-tumor action of schizophyllan, a glucan produced bySchizophyllum Commune.Gann 60, 137 (1969).

    PubMed  CAS  Google Scholar 

  28. Tsugagoshi, S. and Ohashi, F.: Protein-bound polysaccharide preparation, PS-K, effective against mouse sarcoma 180 and rat ascites hepatoma AH-13 by oral use.Gann 65, 557 (1974).

    Google Scholar 

  29. Srivastava, R. and Kulshreshtha, D. K.: Bioactive polysaccharides from plants.Phytochem. 28, 2877 (1989).

    Article  CAS  Google Scholar 

  30. Ohno, N., Sutzuki, I., Oikawa, S., Sato, K., Miyazaki, T. and Yadomae, T.: Antitumor activity and structural charicterization of glucans extracted from cultured fruit bodies ofGrifola frondasa.Chem. Pharm. Bull. 32, 1142 (1984).

    PubMed  CAS  Google Scholar 

  31. Iino, K., Ohno, N., Suzuki, I., Sato, K., Oikawa, S. and Yadomae, T.: Structure-function relationship of antitumor β-1 3-glucan obtained from matted mycelium of culturedGrifola frondosa.Chem. Pharm. Bull. 33, 4950 (1985).

    PubMed  CAS  Google Scholar 

  32. Adachi, Y., Ohno, N., Ohsawa, M., Sata, K., Oikawa, S. and Yadomae, T.: Physicochemical properties and antitumor, activities of chemically modified derivatives of antitumor glucan “Griforan LE” fromGrifola frondosa.Chem. Pharm. Bull. 37, 1838 (1989).

    PubMed  CAS  Google Scholar 

  33. Kang, C. Y., Shim, M. J., Choi, E. C., Lee, Y. N. and Kim, B. K.: Studies on antitumor components of Korean Basidiomycetes. Mycelial culture and antitumor components ofGanoderma lucidum.Kor. Biochem. J. 14, 101 (1981).

    CAS  Google Scholar 

  34. Kim, B. K., Chung, H. S., Chung, K. S. and Yang, M. S.: Studies on the antineoplastic components of Korean Basidiomycetes.Kor. J. Mycol. 8, 107 (1980).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Korea Ginseng and Tobacco Research Institute, 305-345, Taejon, Korea

    Young Sook Kim, Kyu Sang Kang & Shin Il Kim

Authors
  1. Young Sook Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kyu Sang Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shin Il Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, Y.S., Kang, K.S. & Kim, S.I. Study on antitumor and immunomodulating activities of polysaccharide fractions fromPanax ginseng: Comparison of effects of neutral and acidic polysaccharide fraction. Arch. Pharm. Res. 13, 330–337 (1990). https://doi.org/10.1007/BF02858168

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02858168

Keywords

Search

Navigation