Abstract
A procedure utilizing high pressure liquid chromatography coupled with UV detection is described for the determination of blood concentration of higenamine. Deproteinized serum was pretreated with C18 (Sep-pak C18 cartridge) and the 70% EtOH eluent was applied onto a reversed-phase column (μ Bondapak C18) with a 15% acetonitrile in 0.05 N NaH2 PO4-trichloroacetic acid mixed buffer (pH 2.8) as a mobile phase. With the UV detection at 232 nm, the retention times of higenamine and 1,2,3,4-tetrahydropapaveroline, an internal standard, were 5.2 min and 3.9 min respectively. The blood concentration of higenamine was measured at regular intervals afteri.v. injection of higenamine to rabbit. A drastic decrease in higenamine concentration to 30% of the maximum value obtained immediately after the injection, was observed during the first 1–2 min period and thereafter the rate of decrease was slowed down. The analytical result seemed to coincide with the pharmacological effect of higenamine exerting the maximum chronotropic and hypotensive effect at the completion of the injections which were progressively recovered.
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Literature Cited
Xaio, P.: Recent developments on medicinal plants in China.J. Ethnopharmacology 7, 95(1983).
Kosuge, T., Yokota, M. and Nagasawa, M.: Studies on cardiac principle in Aconite roots. I. Isolation and structure determination of higenamine.Yakugaku Zasshi 98, 1370(1978).
Koshiyama, H., Ohkuma, H., Kawaguchi, H., Hsu, H.-T., and Chen, Y.-P.: Isolation of 1-(p-hydroxybenzyl)-6, 7-dihydroxy-1,2,3, 4-tetrahydroisoquinoline (demethylcoclaurine), an active alkaloid fromNelumbo nucifera.Chem. Pharm. Bull. 18, 2564(1970).
Leboeuf, M. and Cave, A.: Isolement de 1’ higenamine a partir de 1’Annona aquamosa: Interet des resines adsorbantes macromoleculaires en chimic vegetale extractive.J. Nat. Prod. 44, 53(1981).
Kosuge, T. and Yokota, M.: Studies on cardiac principle of Aconite root.Chem. Pharm. Bull.,24, 176(1976).
Huang, L.Z., Zhang, D.Y., and Wang, C.Y.: Synthesis of higenamine.Acta Pharmaceutica Sinica 16, 931(1981).
Chang, K.C., Yun-Choi, H.S., Lim, J.-K. and Park, C.-W.: Synthesis of higenamine, A cardiotonic principle of Aconite root.Arch. Pharm. Res. 7, 133(1984).
Feng, Y.-p., Jia, H.-j., Zhang, L.-Y. and Zeng, G.-y.: Effects ofdl-demethyl coclaurine on β-adrenergic receptors and adenylate cyclase in turkey erythrocyte membrane.Acta. Pharm. Sinica 17, 641(1982).
Feng, Y.-P., Zhang, Y. Zhan, H.-s., and Zeng, G.-y.: Effects ofdl-demethylcoclaurine on plasma cyclic AMP in mice.Acta Pharmacol. Sinica 2, 114(1981).
Huang, N.-h., Zhou, Y.-p., Liu, W.-h., Fan, L.-i. and Tseng, K.-y.: Comparison of cardiovascular effects of Aconite root and higenamine in dogs.Acta Pharmacol. Sinica 1, 34(1980).
Han, H.-w., Wang, J.-z. and Sun, F.-l.: Effect ofdl-demethylcoclaurine on cultured rat heart cells.Acta Pharmacol. Sinica 2, 111(1981).
Chen, Z., Liu, L., Zhou, T., Li, Q. and Wang, H.: Effect ofAsarum heterotropoides on the left ventricular function of dogs.Acta Pharmaceutica Sinica 16, 721(1981).
Chang, K.C.: The effect of higenamine upon the interval-strength relationship in isolated rabbit heart.Kor. J. Pharmacol. 19, 9(1983).
Park, C.W., Chang, K.C. and Lim, J.K.: Effects of higenamine on isolated heart adrenoceptor of rabbit.Arch. Int. Pharmacody. Therap. 267, 279(1984).
Kim, N.S., Hong, C.Y., Park, C.W. and Lim, J.K.: An experimental study on the adrenergic effect of higenamine in rabbit cardiovascular system.Kor. Cir. J. 16 1(1986).
Cohen, G.: Alkaloid products in the metabolism of alcohol and biogenic amines.Biochem. Pharmacol. 25, 1123(1976).
Collins, A.C., Cashaw, J.L. and Davis, V.E.: Dopamine derived tetrahydroisoquinoline alkaloids-inhibitors of neuroamine metabolism.Biochem. Pharmacol. 22, 2337(1973).
Mell, L.D. and Gustafson, A.B.: Urinary free norepinephine and dopamine determined by reverse-phase high-pressure liquid chromatography.Clin. Chem. 23, 473(1977).
Moyer, T.P. and Jiang, N.-S.: Optimized isocratic conditions for analysis of catecholamines by high-performance reversed-phase paired-ion chromatography with amperometric detection.J. Chromatogr. 153, 365(1978).
Hjemdahl, P., Daleskog, M. and Kahan, T.: Determination of plasma catecholamines by high performance liquid chromatography with electrochemical detection: Comparison with a radioenzymatic method.Life Science 25, 131(1979).
Yui, Y., Fujita, T., Yamamoto, T., Itokawa, Y. and Kawai, C.: Liquid-chromatographic determination of norepinephrine and epinephrine in human plasma.Clin. Chem. 26, 194(1980).
Davies, C.L. and Molyneux, S.G.: Routine determination of plasma catecholamines using reversed-phase, ion-pair high-performance liquid chromatography with electrochemical detection.J. Chromatogr. 231, 41(1982).
Causson, R.C. and Carruthers, M.E.: Measurement of catecholamines in biological fluids by high-performance liquid chromatography. A comparison of fluorimetric with electrochemical detection.J. Chromatogr. 229, 301(1982).
Kim, J.Q., Kim, K.D. and Jung, H.S.: An optimized assay of plasma catecholamines using high performance liquid chromatography with electrochemical detection.J. Clin. Pathol. Qual. Control. 7, 163(1985).
Wagner, H. and Grevel, J.: Naue herzwirksame Drogen II, Nachweis und Isolierung herzwirksamer Amine durch Ionenpaar-HPLC.Planta Medica 44, 36(1982).
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Park, S.O., Hong, C.Y., Paik, S.W. et al. Determination of blood concentration of higenamine by high pressure liquid chromatography. Arch. Pharm. Res. 10, 60–66 (1987). https://doi.org/10.1007/BF02855622
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DOI: https://doi.org/10.1007/BF02855622