Skip to main content
SpringerLink
Log in

Seeking improved nutritional properties for the potato: Ethionine-resistant protoclones

  • Published:
American Journal of Potato Research Aims and scope Submit manuscript

Abstract

Russet Burbank leaf protoplasts were cultured in Shepard’s (1980) cell layer (CL) medium containing the amino acid analog ethionine (ETN), at concentrations ranging from 0 to 6 µM. Repeated counts of viable cells and dividing colonies during the 21 day incubation period permitted calculation of plating efficiencies for each ETN concentration. Plating efficiency was observed to decrease when ETN concentration in CL exceeded 3 µM. Calli which developed were placed on Shepard’s (1980) C media containing ETN concentrations up to 200 µM; however, few calli survived concentrations above 100 µM. Regenerated plants were grown to maturity and resulting tubers were analyzed for free methionine content. Selected protoclones produced tubers with free methionine content as much as 2.66 times that of the Russet Burbank control.

Resumen

Protoplastos de hojas de Russet Burbank se cultivaron en capa celular de Shepard (1980), medio conteniendo al aminoácido análogo etionina (ETN) en concentraciones que oscilaban entre 0 y 6 M. Contajes repetitivos de células viables y colonias en división durante los 21 días de la incubación de ETN. Se observó que el rendimiento de las plantas disminuyó cuando las concentraciones de ETN excedían los 3M.

Los callos que desarrollaron fueron colocados en medio C Shepard conteniendo concentraciones hasta 200 M de ETN; sin embargo, algunos callos sobrevivieron a concentraciones por encima de los 100M.

Las plantas regneradas se dejaron crecer hasta la madurez y los tubérculos resultantes fueron analizados para su contenido de metionina. Los protoclones seleccionados produjeron tubérculos con un contenido de metionina libre de más de 2,66 que el control de Russet Burbank.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  • Butenko, R.G., A.A. Kucho, V.A. Vitenko, and V.A. Avetison. 1977. Production and cultivation of isolated protoplasts from the mesophyll of leavesof Solanum tuberosum L. andSolanum chaconse Bitt.. Soviet Plant Pathol 24:660–665.

    CAS  Google Scholar 

  • Carlson, P.S. 1973. Methionine sulfoximine-resistant mutants of tobacco. Science 180:1366–1368.

    Article  PubMed  CAS  Google Scholar 

  • Carlson, J.E. and J.M. Widholm. 1978. Separation of two forms of anthranilate synthetase from 5-methltryptophan susceptible and resistantSolanum tuberosum cells. Physiol Plant 44:251–260.

    Article  CAS  Google Scholar 

  • Curtiss, C.D., J.M. Widholm and R.A. Gonzales. 1987. Selection and characterization of methionine and tryptophan analog resistant asparagus cells. J Plant Physiol 130:125–135.

    CAS  Google Scholar 

  • Desborough, S.L. 1985. Potato proteins.In: Potato Physiology. P.H. Li. ed. Academic Press, Orlando. pp. 329–351.

    Google Scholar 

  • DeVries, S.E. and G.S. Bokelmann. 1986. Regeneration of calli and plants from cell suspension protoplasts of dihaploid potatoes. J Plant Physiol 122:199–203.

    CAS  Google Scholar 

  • Forsline, P.L. and A.R. Langille. 1976. An assessment of the modifying effect of kinetin on thein vitro tuberization of induced and noninduced tissues ofSolanum tuberosum. Can J Bot 54:2513–2516.

    Article  CAS  Google Scholar 

  • Gonzales, R.A., P.K. Das, and J.M. Widholm. 1984. Characterization of cultured tobacco cell lines resistant to ethionine, a methionine analog. Plant Physiol 74:640–644.

    PubMed  CAS  Google Scholar 

  • Gustine, D.L. 1984. Versatile microcomputer-controlled, automated gradient analytical high-performance liquid chromatography system. J Chromatography 316:407–414.

    Article  CAS  Google Scholar 

  • Gustine, D.L. 1985. Determination of s-methyl cysteine sulfoxide in Bras-sica extracts by high-performance liquid chromatography. J Chromatography 319:450–453.

    Article  CAS  Google Scholar 

  • Horton, D. and R.L. Sawyer. 1985. The potato as a world food crop, with special reference to developing areas.In: Potato Physiology. P.H. Li. ed. Academic Press, Orlando. pp: l-34.

    Google Scholar 

  • Jacobsen, E., R.G. F. Visser, and J. Wijbrandi. 1985. Phenylalanine and tyrosine accumulating cell lines of a dihaploid potato selected for resistance to 5-methyltryptophan. Plant Cell Rpt 5:151–154.

    Article  Google Scholar 

  • Johnson, V.A. and C.L. Lay. 1974. Genetic improvement of plant protein. Agric Food Chem 22: 558–566.

    Article  CAS  Google Scholar 

  • Kaldy, M.S. 1972. Protein yield of various crops as related to protein value. Econ Bot 26:142–144.

    Google Scholar 

  • Kaldy, M.S. and P. Markakis. 1972. Amino acid composition of selected potato varieties. J Food Sci 37:375–377.

    Article  CAS  Google Scholar 

  • Langille, A.R. and K.L. Prouty. 1987. An evaluation of the effect of three cytokinins on plating efficiency in protoplast populations of potato. HortScience 22(1): 135–136.

    CAS  Google Scholar 

  • Luescher, R. 1972. Genetic variability of “available” methionine, total protein, specific gravity and other traits in tetraploid potatoes. PhD thesis. Michigan State University.

  • Mulder, E.G. and K. Bakema. 1956. Effect of nitrogen, phospohorus and magnesium nutrition of potato plants on the content of free amino acids and on the amino acid compositions of the protein of tubers. Plant Soil 7:135–166.

    Article  CAS  Google Scholar 

  • Reisch, B., S.H. Duke, and E.T. Bingham. 1981. Selection and characterization of ethionine-resistant alfalfa (Medicago sativa L.) cell lines. Theor Appl Genet 59:89–94.

    Article  Google Scholar 

  • Rexen, B. 1976. Studies of protein of potatoes. Potato Res 19:189–202.

    Article  CAS  Google Scholar 

  • Shepard, J.F. 1980. Mutant selection and plant regeneration from potato mesophyll protoplasts.In: Genetic improvement of crops. Emergent techniques. I. Rubenstein, B. Genegenbach, R.L. Phillips and C.E. Green, eds. University of Minnesota Press, Minneapolis.

    Google Scholar 

  • Talley, E.A. and M.J. Egoville. 1988. Extraction of free amino acids. Am Potato J 65:17–20.

    CAS  Google Scholar 

  • Tu, H.M., L.W. Godfrey, and S.M. Sun. 1994. Expression of the Brazil nut methionine-rich protein in transgenic potato plants. pp. 209–220.In: The molecular and cellular biology of the potato. 2nd ed. Belknap, W.R., M.E. Vayda and W.D. Park, eds. CAB International, Wallingford, UK

    Google Scholar 

  • Widholm, J.M. 1976. Selection and characterization of cultured carrot and tobacco cells resistant to lysine, methionine, and proline analogs. Can J Bot 54:1523–1529.

    CAS  Google Scholar 

  • Widholm, J.M. 1977. Selection and characterization of amino acid resistant plant cell cultures. Crop Sci 17:597–600.

    Article  CAS  Google Scholar 

  • Woolfe, J.A. 1986a The Potato in the Human Diet. Cambridge University Press, Cambridge, UK pp 58–82.

    Google Scholar 

  • Woolfe, J.A. 1986b. Potato- a gift from the Andes. Brit Nut Fdn Bull 46 11: 29–45.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biosystems Science and Engineering, University of Maine, 04469, Orono, ME

    Alan R. Langille (Professor)

  2. Jackson Laboratory, 04609, Bar Harbor, ME

    Yu Lan (Res. Technician and former graduate assistant)

  3. USDA/ARS Pasture Lab., 16802, University Park, PA

    D. L. Gustine (Res. Plant Physiologist)

Authors
  1. Alan R. Langille
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yu Lan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. L. Gustine
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Alan R. Langille.

Additional information

Maine Agricultural and Forest. Experiment Station Publication No. 2241.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Langille, A.R., Lan, Y. & Gustine, D.L. Seeking improved nutritional properties for the potato: Ethionine-resistant protoclones. Am. J. Pot Res 75, 201–205 (1998). https://doi.org/10.1007/BF02854213

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02854213

Additional Key Words

Search

Navigation