Abstract
Quantitative differences among strains ofCorynebacterium sepedonicum (Spieck. and Kotth.) Skapt. and Burkh. (=Clavibacter michiganense subsp.sepedonicum) were distinguished in eggplant (Solanum melongena L. ‘Black Beauty’) bioassays with a simple leaf axil inoculation technique. Latent period of infection (time from inoculation to first appearance of symptoms) varied inversely with inoculum dose. Latent period of infection varied significantly among strains ofC. sepedonicum. Symptoms developed more rapidly in plants grown under controlled conditions in a growth chamber than those grown in a greenhouse. Plants treated with inoculum prepared from broth cultures exhibited symptoms more rapidly than those treated with inoculum grown on agar.
Resumen
Se encontraron diferencias cuantitativas entre las variantes de Corynebacterium sepedonicum (Spieck y Kotth) Skapt. y Burkh. (=clavibacter michiganense) subsp.sepedonicum, al efectuar pruebas biológicas utilizando la prueba simple de inoculación de la bacteria en la axila de las hojas de plantas de berenjena (Solanum melongena L. ‘Black Beauty’). El período latente de infección (tiempo transcurrido desde la inoculación hasta la aparición de los primeros síntomas) varió en relación inversa con la dosis del inóculo. El período latente de infección varió significativamente entres las variantes deC. sepedonicum. Los síntomas se desarrollaron más rápidamente en las plantas creciendo bajo condiciones controladas en una cámara de crecimiento que en aquellas que crecían en un invernadero.
Las plantas tratadas con inóculo obtenido en caldo de cultivo mostraron síntomas más rápidamente que aquellas tratadas con inóculo producido sobre agar.
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Former Graduate Research Assistant
This work was supported in part by the College of Agriculture and Life Sciences, University of Wisconsin-Madison, and by a grant from the Wisconsin Potato Industry Board.
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Bishop, A.L., Slack, S.A. Effect of inoculum dose and preparation, strain variation, and plant growth conditions on the eggplant assay for bacterial ring rot. American Potato Journal 64, 227–234 (1987). https://doi.org/10.1007/BF02853560
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DOI: https://doi.org/10.1007/BF02853560