Abstract
The latex agglutination test was evaluated for detection of the potato bacterial ring rot pathogen,Corynebacterium sepedonicum. The bacterium was detected in infected potato stems and tubers tested and in inoculated eggplant stems. Test reliability was confirmed using blind tests. A minimum of 106 bacterial cells/ml was requisite for observable agglutination. Nonspecific reactions were not observed from healthy extracts of plant tissues. Nonspecific reactions with other bacteria maintained in pure culture were eliminated by addition of bovine serum albumin to sensitized latex preparations.
Resumen
La aglutinación de látex fue evaluada como prueba para la detección deCorynebacterium sepedonicum, agente etiológico de la podredumbre anular en la papa. Tallos y tubérculos de papas infectadas, así como tallos de berengena previamente inoculados se mostaron positivas a la prueba. Las muestras fueron previamente codificadas, para que el individuo que realizó la prueba desconociera su procedencia (prueba a ciegas). Para observar la aglutinación de látex fue necesario tener una cantidad mínima de 106 bacterias/ml. No se observaron reacciones inespecíficas contra extractos de tejidos vegetales sanos. Las reacciones inespecíficas contra cultivos puros, de otras bacterias fueron eliminadas al agregar albumina bovina a la solución amortiguadora con el látex sensibilizado.
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This research was supported by the College of Agricultural and Life Sciences, University of Wisconsin-Madison.
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Slack, S.A., Sanford, H.A. & Manzer, F.E. The latex agglutination test as a rapid serological assay forCorynebacterium sepedonicum . American Potato Journal 56, 441–446 (1979). https://doi.org/10.1007/BF02854063
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DOI: https://doi.org/10.1007/BF02854063