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The effect of fluorescent labeling on calcium-induced fusion of fusogenic carrot protoplasts

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Abstract

Various fluorescent compounds — carboxyfluorescein, scopoletin, fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), rhodamine 123, and rhodamine B ethyl ester — were used to study their effects on calcium-induced fusion of fusogenic carrot (Daucus carota L.) protoplasts. These protoplasts normally fused at a high percentage (50–60%) in response to 10 mM calcium, pH 6.0; however, if cells had been labeled with scopoletin, FITC, or RITC, fusion was greatly reduced. In contrast, labeling with carboxyfluorescein, rhodamine 123, or rhodamine B ethyl ester had no detectable effect on calcium-induced fusion. The two rhodamine dyes are shown to be localized in mitochondria.

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Abbreviations

EGTA:

ethyleneglycol-bis-(β-aminoethyl ether) N′,N′-tetraacetic acid

FITC:

fluorescein isothiocyanate

RITC:

rhodamine isothiocyanate

PE:

phosphatidylethanolamine

References

  • Boss, WF, Kelly CJ, Landsberger FR (1975) Anal Biol 64: 289–292

    Google Scholar 

  • Boss, WF, Grimes HD, Brightman AO (1984) Protoplasma 120: 209–215

    Google Scholar 

  • Deutscher SL, Creek KE, Morre DJ (1981) J Cell Biol 25: 324–330

    Google Scholar 

  • Galbraith DW (1984) In: Vasil IK (ed) Cell Culture and Somatic Cell Genetics of Plants, Vol 1, Academic Press, NY, pp 433–447

    Google Scholar 

  • Galbraith DW, Mauch TJ (1980) Z Pflanzenphysiol 98: 129–140

    Google Scholar 

  • Johnson, LV, Walsh ML, Chen LB (1980) Proc Nat Acad Sci 77: 990–994

    Google Scholar 

  • Johnson, LB, Walsh ML, Bockus BJ, Chen LB (1981) J Cell Biol 88: 526–535

    Google Scholar 

  • Kanchanapoom K, Brightman AO, Grimes HD, Boss WF (1985) Protoplasma 124: 65–70

    Google Scholar 

  • Meadows MG, Potrykus I (1981) Plant Cell Rep 1: 77–79

    Google Scholar 

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Authors and Affiliations

  1. Department of Botany, North Carolina State University, 27695-7612, NC, USA

    Kamnoon Kanchanapoom & Wendy F. Boss

Authors
  1. Kamnoon Kanchanapoom
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  2. Wendy F. Boss
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Communicated by J. M. Widholm

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Kanchanapoom, K., Boss, W.F. The effect of fluorescent labeling on calcium-induced fusion of fusogenic carrot protoplasts. Plant Cell Reports 5, 252–255 (1986). https://doi.org/10.1007/BF00269814

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  • DOI: https://doi.org/10.1007/BF00269814

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