Abstract
A sulfated galactan composed of nearly equimolar amounts of d-galactose, 3,6-anhydro-d-galactose, and sulfate was isolated from the red alga Turnerella mertensiana collected in the Sea of Japan. The structures of native polysaccharide and its alkaline modification products were studied by NMR spectroscopy. The polysaccharide molecules were shown to contain a linear carbohydrate chain consisting of alternating 3-linked β-d-galactopyranose 4-sulfate and 4-linked 3,6-anhydro-α-d-galactopyranose residues (known as к-carrageenan), which is typical of carrageenans, but the regularity of polymer structure is masked by the presence of some 3,6-anhydro-α-d-galactose 2-sulfate (ι-carrageenan units) and α-D-galactose 6-sulfate (µ-carrageenan units) instead of 3,6-anhydro-α-d-galactose. Upon addition of potassium chloride (up to 4%) to a solution of the native polysaccharide, about half of the substance transforms into gel. The gel-forming fraction is к-ι-µ-hybrid carrageenan with the ∼65 : 15 : 20 ratio of к-, ι-, and µ-units. The non-gelling fraction contains the к-, ι-, and µ-units at the ratio of ∼46 : 12 : 42. The gel-forming carrageenan product free of µ-units can be otained in ∼30% yield (based on the dry biomass) by alkaline treatment of the alga prior to extraction of the polysaccharide.
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For Part 66, see Ref. 1.
On the occasion of the 100th anniversary of the birth of Academician N. K. Kochetkov (1915—2005) and the 50th anniversary of the G. B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far Eastern Branch of the Russian Academy of Sciences.
Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 5, pp. 1163—1167, May, 2015.
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Vaskovsky, V.E., Smirnova, G.P., Shashkov, A.S. et al. Polysaccharides of algae 67. Carrageenan from Pacific red alga Turnerella mertensiana (Gigartinales, Rhodophyta). Russ Chem Bull 64, 1163–1167 (2015). https://doi.org/10.1007/s11172-015-0993-4
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DOI: https://doi.org/10.1007/s11172-015-0993-4