Abstract
A high frequency of type II restriction endonuclease activities was detected inSelenomonas ruminantium but not in other rumen bacteria tested. Eight different restriction endonucleases were characterized in 17 strains coming from genetically homogeneous local population. Chromosomal DNA isolated fromS. ruminantium strains was found to be refractory to cleavage by various restriction enzymes, implying the presence of methylase activities additional to those required for protection against the cellular endonucleases. The presence of Dam methylation was detected inS. ruminantium strains as well as in several other species belonging to theSporomusa subbranch of low G+C Gram-positive bacteria (Megasphaera elsdenii, Mitsuokella multiacidus).
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References
Hemavathy K.C., Nagaraja V.: DNA methylation in mycobacteria: absence of methylation at GATC (Dam) and CCA/TGG (Dcm) sequences.FEMS Immunol. Med. Microbiol.11, 291–296 (1995).
Maniatis T., Fritsch E.F., Sambrook J.:Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1982.
Marmur J.: A procedure for the isolation of deoxyribonucleic acid from microorganisms.J. Mol. Biol.3, 208–218 (1961).
Molnárová V., Javorský P. Pristaš P.: Prevalence of CTGCAG recognizing restriction and modification systems in ruminal selenomonades.Anaerobe5, 37–41 (1999).
Noyer-Wiedner M., Trautner T.A.: Methylation of DNA in prokaryotes, pp. 39–108 in J.P. Jost, H.P. Saluz (Eds):DNA Methylation: Molecular Biology and Biological Significance. Birkhauser Verlag, Basle 1993.
Pristaš P., Molnárová V., Javorský P.: Detection ofN6-methyladenine in GATC sequences ofSelenomonas ruminantium.J. Basic Microbiol.38, 283–287 (1998).
Pristaš P., Vanat I., Javorský P.: Variability of endonucleolytic activity indicates high genetic diversity among natural populations ofSelenomonas ruminantium.Folia Microbiol.42, 121–124 (1997).
Štyriak I., Pristaš P., Javorský P.: Lack of surface receptors not restriction-modification system determines F4 phage resistance inStreptococcus bovis II/1.Folia Microbiol.44, 35–38 (1998).
Wilson G.G., Murray N.E.: Restriction and modification systems.Ann. Rev. Genet.25, 585–627 (1991).
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Pristaš, P., Molnárová, V. & Javorský, P. Restriction and modification systems of ruminal bacteria. Folia Microbiol 46, 71–72 (2001). https://doi.org/10.1007/BF02825890
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DOI: https://doi.org/10.1007/BF02825890