Summary
Production of transgenic maize (Zea mays L.) callus, plants, and progeny from microprojectile bombardment of 2–5-d cultured Hi-II immature embryos is described. Histological evidence indicates that these tissues are amenable to transformation due to surface layer cell division of the scutellum. Two out of every 100 bombarded embryos produced transgenic callus and R0 transgenic plants were both male and female fertile. Expected segregation of transgenes was observed in progeny. The primary advantage of bombarding these tissues is increased male and female fertility of transgenic plants compared with those produced using long-term callus or suspension cultures.
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References
Armstrong, C. L. Somatic cell regeneration systems for in vitro genetic manipulation. In: Freeling, M.; Walbot, V., eds. The maize handbook. New York: Springer-Verlag; 1993:663–671.
Armstrong, C. L.; Green, C. E. Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline. Planta 164:207–214; 1985.
Armstrong, C. L.; Green, C. E.; Phillips, R. L. Development and availability of germplasm with high type II culture formation response. Maize Genetics Cooperation Newsletter 65:92–93; 1991.
Armstrong, C. L.; Songstad, D. D. Method for transforming monocotyledonous plants. European Patent Application Number 93870173.7; 1993.
Callis, J.; Fromm, M.; Walbot, V. Introns increase gene expression in cultured maize cells. Genes & Dev. 1:1183–1200; 1987.
Casas, A. M.; Kononowicz, A. J.; Zehr, U. B., et al. Transgenic sorghum plants via microprojectile bombardment. Proc. Natl. Acad. Sci. 90:11212–11216; 1993.
Christou, P.; Ford, T. L.; Kofron, M. Production of transgenic rice (Oryza sativa L.) plants from agronomically important indica and japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos. Bio/Technol. 9:957–962; 1991.
Chu, C. C.; Wang, C. C.; Sun, C. S., et al. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci. Sin. 18:659–668; 1975.
Cooley, J.; Ford, T.; Christou, P. Molecular and genetic characterization of elite transgenic rice plants produced by electric-discharge particle acceleration. Theor. Appl. Genet. 90:97–104; 1995.
D'Halluin, K.; Bonne, E.; Bossut, M., et al. Transgenic maize plants by tissue electroporation. Plant Cell 4:1495–1505; 1992.
Dunder, E.; Dawson, J.; Brewer, J., et al. High frequency transformation of maize by microprojectile bombardment of immature embryos. In Vitro Cell. Dev. Biol. 29A:83; 1993.
Fromm, M. E.; Morrish, F.; Armstrong, C., et al. Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio/Technol. 8:833–839; 1990.
Gordon-Kamm, W. J.; Spencer, T. M.; Mangano, M. L., et al. Transformation of maize cells and regeneration of fertile transgenic plants. Plant Cell 2:603–618; 1990.
Hagio, T.; Hirabayashi, T.; Machii, H., et al. Production of fertile transgenic barley (Hordeum vulgare L.) plant using the hygromycin-resistance marker. Plant Cell Rep. 14:329–334; 1995.
Haughn, G. W.; Smith, J.; Mazur, B., et al. Transformation with a mutant Arabidopsis acetolactate synthase gene renders tobacco resistant to sulfonylurea herbicides. Mol. & Gen. Genet. 211:266–271; 1987.
Jefferson, R. A.; Kavanagh, T. A.; Bevan, M. W. Gus fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6:3901–3907; 1987.
Klein, T. M.; Gradziel, T.; Fromm, M. E., et al. Factors influencing gene delivery intoZea mays cells by high-velocity microprojectiles. Bio/Technol. 6:559–563; 1988.
Koziel, M. G.; Beland, G. L.; Bowman, C., et al. Field performance of elite transgenic maize plants expressing an insecticidal protein derived fromBacillus thuringiensis. Bio/Technol. 11:194–200; 1993.
Lee, K. Y.; Townsend, J.; Tepperman, J., et al. The molecular basis of sulfonylurea herbicide resistance in tobacco. EMBO J. 7:1241–1248; 1988.
Li, B. J.; Xu, X. P.; Shi, H. P., et al. Introduction of foreign genes into the seed embryo, cells of rice by electroinjection and the regeneration of transgenic rice plants. Sci. China 34:923–930; 1991.
Li, L.; Qu, R.; de Kochko, A., et al. An improved rice transformation system using the biolistic method. Plant Cell Rep. 12:250–255; 1993.
Perl, A.; Kless, H.; Blumenthal, A., et al. Improvement of plant regeneration and GUS expression in scutellar wheat calli by optimization of culture conditions and DNA-microprojectile delivery procedures. Mol. & Gen. Genet. 235:279–284; 1992.
Peschke, V. M.; Phillips, R. L. Genetic implications of somaclonal variation in plants. Adv. Genet. 30:41–75; 1992.
Ritala, A.; Aspegren, K.; Kurten, U., et al. Fertile transgenic barley by particle bombardment of immature embryos. Plant Mol. Biol. 24:317–325; 1994.
Rochester, D. E.; Winters, J. A.; Shah, D. M. The structure and expression of maize genes encoding the major heat shock protein. EMBO J. 5:451–458; 1986.
Songstad, D. D.; Armstrong, C. L.; Petersen, W. L. AgNO3 increases type-II callus production from immature embryos of maize inbred B73 and its derivatives. Plant Cell Rep. 9:699–702; 1991.
Songstad, D. D.; Halaka, F. G.; DeBoer, D. L., et al. Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electroporation. Plant Cell Tissue Organ Cult. 33:195–201; 1993.
Walters, D. A.; Vetsch, C. S.; Potts, D. E., et al. Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants. Plant Mol. Biol. 18:189–200; 1992.
Wan, Y.; Lemaux, P. G. Generation of large numbers of independently transformed fertile barley plants. Plant Physiol. 104:37–48; 1994.
Weeks, J. T.; Anderson, O. D.; Blechl, A. E. Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiol. 102:1077–1084; 1993.
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Songstad, D.D., Armstrong, C.L., Petersen, W.L. et al. Production of transgenic maize plants and progeny by bombardment of hi-II immature embryos. In Vitro Cell Dev Biol - Plant 32, 179–183 (1996). https://doi.org/10.1007/BF02822763
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DOI: https://doi.org/10.1007/BF02822763