Abstract
Strains ofM. ciceri, symbionts of chickpea (Cicer arietinum) were incubated with the flavonoids naringenin, daidzein and quercetin which have earlier been reported as inducers and inhibitors ofnodABC-lacZ fusion ofM. ciceri. Preincubation ofM. ciceri with naringenin and daidzein (100 nmol/L) for 1 d improved the competitive ability of the inoculated strain while preincubation with quercetin decreased the nodule occupancy of inoculated strain under sterile conditions. Under nonsterile conditions induced strains of Rcd 301 and HT-6 formed by 23 and 18% more nodules, respectively, than untreated control. Quercetin-treated strains showed by 13–20% fewer nodules than untreated controls. Therefore, it is possible to regulate the competitive ability of inoculated strains by flavonoid treatment.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Belkheir A.M., Zhou X., Smith D.L.: Variability in yield and yield component response to genestein pre-incubationBradyrhizobium japonicum by soybean (Glycine max (L.)Merr.) cultures.Plant & Soil229, 41–46 (2001).
Cunningham S., Kollmeyer W.D., Stacey G.: Chemical control of nodulation byBradyrhizobium japonicum.Appl. Environ. Microbiol.57, 1886–1892 (1991).
Dahiya J.S., Khurana A.L.: Chillum jar, a better technique for screening rhizobia under summer conditions.Plant & Soil63, 299–302 (1981).
Denarie J., Debelle F., Prome J.C.:Rhizobium lipo chito oligosaccharide nodulation factors: signalling molecules mediation, recognition and morphogenesis.Ann. Rev. Biochem.65, 503–535 (1996).
Gaur Y.D., Sen A.N.: Cross inoculation group specificity inCicer-Rhizobium symbiosis.New Phytol.83, 745–754 (1979).
Hungria M., Joseph C.M., Phillips D.A.:Rhizobium nod gene inducer exuded naturally from roots of common bean (Phaseolus vulgari).Plant Physiol.97, 759–764 (1991).
Jain V., Nainawatee H.S.: Flavonoids influence growth and saccharide metabolism ofRhizobium meliloti.Folia Microbiol.44, 311–316 (1999).
Jain V., Garg N., Nainawatee H.S.: Influence of flavonoids on nodulation and carbon-partitioning in pea-Rhizobium symbiosis.J. Plant Biochem. Biotechnol.1, 23–26 (1992).
Kamboj D.V., Sharma P.K., Kundu B.S.: Direct monitoring ofRhizobium sp. (Cicer) in nodule and soil usinglacZ fusions.Biol. Fertil. Soil21, 309–313 (1996).
Khurana A.L., Sharma P.K., Dudeja S.S.: Influence of host, moisture and native rhizobial population on nodule occupancy of chickpea (Cicer arietinum).Zbl. Bakteriol.146, 137–141 (1991).
Kingley M.T., Bohlool B.B.: Characterization ofRhizobium sp. (Cicer) by immunofluorescence, immunodiffusion and intrinsic antibiotic resistance.Can. J. Microbiol.29, 518–526 (1983).
Kosslak R.M., Bookland R., Barbei J., Paaren H.E., Appelbaum E.R.: Induction ofBradyrhizobium japonicum commonnod genes by isoflavones isolated fromGlycine max.Proc. Nat. Acad. Sci. USA84, 7428–7432 (1987).
Kosslak R.M., Joshi R.S., Bowen B.A., Paaren H.E., Appelbaum E.R.: Strain specific inhibition ofnod genes inBradyrhizobium japonicum by flavonoid compounds.Appl. Environ. Microbiol.56, 1333–1341 (1990).
Long S.R.:Rhizobium-legume symbiosis: Nod factors in prespectives.Plant Cell8, 1885–1898 (1996).
McIver J., Djordjević M.A., Weenmann J.T., Bender G.L., Rolfe B.G.: Extension of host range ofRhizobium leguminosarum bv.trifolii caused by point mutations innodD that results in alternation in regulatory function and recognition of inducer molecules.Mol. Plant Microbe Interact.2, 97–106 (1989).
Mikiashevisch E., Rohrig H., Schell J., Schmidt J.: Perception and signal transduction of rhizobial NOD factors.Crit. Rev. Plant Sci.20, 373–394 (2001).
Peter N.K., Frost J.W., Long S.R.: A plant flavone leutolin induce expression ofR. melilori nodulation genes.Science233, 977–980 (1986).
Redmond J.W., Batley M., Djordjevií M.A., Innes R.W., Kuempel P.L., Rolfe B.G.: Flavone induced expression of nodulation genes inRhizobium.Nature323, 632–635 (1986).
Relić B., Perret X., Estrada-Garcia M.T., Koprinowki W., Krishanan H.B., Puppeke S.G., Broughton W.J.: Nod factors ofRhizobium are key to legume door.Mol. Microbiol.13, 171–178 (1994).
Saini V., Sindhu S.S., Dadarwal K.R.: Azide-resistant mutants ofAzorhizobium caulinodans with enhanced symbiotic effectivenes.Folia Microbiol.46, 217–222 (2001).
Sharma P.K., Lakshminarayana K.: Effect of high temperature plasmid curing ofRhizobium sp. (Cajanas) in relation to nodulation in pigeonpea (Cajanas cajanL. Millsp.).Biol. Fertil. Soil8, 73–79 (1989).
Sharma P.K., Anand R.C., Lakshminarayana K.: Construction of Tn5 tagged mutants ofRhizobium sp. (Cicer) for ecological studies.Soil Biol. Biochem.23, 881–885 (1991).
Sharma P.K., Kamboj D.V., Rustogi N., Dogra R.C.: A simple visual method to study nodule occupancy ofRhizobium marked withgusA.Ind. J. Microbiol.37, 91–94 (1997).
Sharma P.K., Kundu B.S., Dogra R.C.: Molecular mechanism of host specificity in legume-Rhizobium symbiosis.Biotech. Adv.11, 741–779 (1993).
Sharma P.K., Upadhayay K.K., Srivastva P.: Nodulation and nitrogen fixation ability of wild species ofCicer.Legume Res.17, 229–230 (1994).
Spaink H.P.: Root nodulation and infection factors produced by rhizobial bacteria.54, 257–288 (2000).
Srivastava P., Sharma P.K., Dogra R.C.: Inducers ofnod genes ofRhizobium sp. (Cicer).Microbiol. Res.154, 49–54 (1997).
Triplett E.W.: Isolation of genes involved in nodulation competitiveness fromRhizobium leguminosarum bv.trifolii T24.Proc. Nat. Acad. Sci. USA85, 3810–3814 (1988).
Vincent J.M.: A manual for the practical study of the root nodule bacteria, pp. 75–76 inI.B.P. Handbook no. 15. Blackwell Scientific Publication, Oxford 1970.
Upadhyay K.K., Kamboj D.V., Sharma P.K.: Host specificity of nodulation inRhizobium sp. (Cicer) as revealed byTsr bioassay.Folia Microbiol.42, 381–384 (1997).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sharma, P.K., Upadhyay, K.K., Kamboj, D.V. et al. Preincubation ofMesorhizobium ciceri with flavonoids improves its nodule occupancy. Folia Microbiol 47, 541–544 (2002). https://doi.org/10.1007/BF02818795
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02818795