Abstract
Bioassay based on thick and short root (Tsr) and hair deformation (Had) phenotypes were used to test the activity of Nod factors produced byRhizobium sp. (Cicer) strains HS-1, Rcd-301, IC-59, IC-76 and Ca-181 on chickpea (Cicer arietinum) cv. ‘C-235’. Nod− mutants ofRhizobium sp. (Cicer) did not produce Tsr+ and Had+ phenotypes on chickpea, indicating the requirement of nodulation genes for their appearance. The strain HS-1 treated with root exudates of pea (Pisum sativum), berseem (Trifolium alexandrinum) and lucerne (Medicago sativa) failed to produce the Tsr+ and Had+ phenotypes on chickpea. ConverselyR. leguminosarum bvs.viciae andtrifolii, R. meliloti, Rhizobium sp. (Sesbania), andRhizobium sp. (Cajanus) induced with chickpea root exudates did not show Tsr+ and Had+ phenotypes on chickpea. It appears that host specificity inRhizobium sp. (Cicer)-chickpea symbiosis is regulated by the production of host-specific factors which are not active on heterologous hosts.
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Upadhyay, K.K., Kamboj, D.V. & Sharma, P.K. Host specificity of nodulation inRhizobium sp. (Cicer) as revealed by Tsr bioassay. Folia Microbiol 42, 381–384 (1997). https://doi.org/10.1007/BF02816953
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DOI: https://doi.org/10.1007/BF02816953