Abstract
Asparaginase production by a mesophilic strain ofErwinia sp. was examined; the maximum of activity was found at 40°C and pH 8.5. Among the various carbon sources, mannitol was shown to be the best for production of activity. Inorganic nitrogen sources were better than the organic ones. The enzyme activity was not inhibited by 10 mmol/L metal ions (Na+, K+, Mg2+, Ca2+, Ba2+, Co2+, Ni2+, Zn2+); the activity was strongly inhibited by addition of EDTA.l-Arginine,dl-alanine,l-asparagine andl-glutamine stimulated thel-asparaginase production by 3.9, 1.7, 4.3 and 4.0 fold, respectively. The combination ofl-arginine,l-asparagine andl-glutamine synergistically stimulated the asparaginase up to 5.8 fold.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alper H.O., Lewis D.A.: Therapeutic efficacy of asparaginase encapsulated in intact erythrocytes.Biochem. Pharmacol.,34, 257–261 (1985).
Boyse A.E., Old L.J., Campbell H.A., Mashburn L.T.: Suppression of murine leukemia of various types, comparative inhibitory activities of guinea pig seruml-asparaginase andE. coli l-asparaginase.J. Exp. Med. 125, 17–31 (1967).
Broome J.D.: Evidence that thel-asparaginase activity of guinea pig serum is responsible for its antilymphoma effects.Nature.191, 1114–1115 (1961).
Broome J.D.: Factorw which influence the effectiveness ofl-asparaginase as tumor inhibitors.Brit. J. Cancer. 22, 595–602 (1968).
Campbell H.A., Mashburn L.T., Boyse E.A., Old L.J.: Twol-asparaginases fromE. coli B: their separation, purification and antitumor properties.Biochemistry 6, 721–730 (1967).
Clesceri L.S., Greenberg A.E., Trussell R.R.:Standard Methods of Examination of Water and Wastewater, 17th ed., pp. 4117–4120. American Public Health Association, Washington (DC) 1989.
Kase H., Nakayama K.: Mechanism ofl-threonine andl-lysine production by analog-resistant mutants ofCorynebacterium glutamicum.Agr. Biol. Chem.,38, 993–1000 (1974).
Mohapatra B.R., Bapuji M., Banerjee U.C.: Production and properties ofl-asparaginase fromMucor species associated with a marine sponge (Spirastrella sp.).Cytobios,92, 165–173 (1997).
Mohapatra B.R., Sani R.K., Banerjee U.C.: Characterization ofl-asparaginase fromBacillus sp. isolated from an intertidal marine alga (Sargassum sp.).Lett. Appl. Microbiol. 21, 380–383 (1995).
Mukherjee J., Joeris K., Riechel P., Scheper T.: A simple method for the isolation and purification ofl-asparaginase fromEnterobacter aerogenes.Folia Microbiol. 44, 15–19 (1999).
Raha S.K., Roy S.K., Dey S.K., Chakrabarty S.L.: Purification and properties ofl-asparaginase fromCylindrocarpon obtusisporum.Biochem. Internat.,21, 987–1000 (1990).
Selvakumar Vanajakumar N., Natarajan R.: Partial purification, characterization and antitumor properties ofl-asparaginase (antileukemic agent) from a marineVibrio, pp. 289–300 in M.F. Thompson, R. Sarojini, R. Nagabhushanam (Eds):Indo-US Symp. Bioactive Compounds from Marine Organisms. Oxford & IBH Publishers, New Delhi (India) 1989.
Stepanyan K.R., Davtyan M.A.: Some questions of themostability ofl-asparaginase of the yeastCandida guilliermondii.Biolog Zh. Armenii 41, 599–603 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Borkotaky, B., Bezbaruah, R.L. Production and properties of asparaginase from a newErwinia sp.. Folia Microbiol 47, 473–476 (2002). https://doi.org/10.1007/BF02818783
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02818783