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A simple method for the isolation and purification ofl-asparaginase fromEnterobacter aerogenes

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Abstract

l-Asparaginase fromEnterobacter aerogenes was purified by a simple method involving sonication of the crude cell mass, gel filtration with Sephacryl S-100 as the separating material, followed by ultrafiltration. Recent methods involve complex purification procedures of 5–6 steps. The isolation process resulted in 10-fold purification of the enzyme with a specific activity of 55 IU/mg protein and recovery of 54%. The purity was tested by capillary electrophoresis, used for the first time for documenting the purification ofl-asparaginase. The choice of the column material was critical in the purification process.

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Authors and Affiliations

  1. Institut für Technische Chemie der Universität Hannover, 30167, Hannover, Germany

    J. Mukherjee, K. Joeris, P. Riechel & T. Scheper

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  1. J. Mukherjee
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  2. K. Joeris
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  3. P. Riechel
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  4. T. Scheper
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Correspondence to T. Scheper.

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Mukherjee, J., Joeris, K., Riechel, P. et al. A simple method for the isolation and purification ofl-asparaginase fromEnterobacter aerogenes . Folia Microbiol 44, 15–18 (1999). https://doi.org/10.1007/BF02816214

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  • DOI: https://doi.org/10.1007/BF02816214

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