Abstract
Protective effect of the lipopolysaccharide (LPS) antigen ofKlebsiella pneumoniœ was tested against ascending-mode urinary tract infection in BALB/c and LACA strains of mice. LPS was given by two different routes; LPS was found to be protective (whatever the application route) since colonization with the challenge organism was significantly lower in both cases as compared with unimmunized mice. A maximum decrease in bacterial count in the kidney of LPS-treated animals was observed on challenge after a 4-d treatment.
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References
Burton K.: Diphenylamine method for DNA estimation.Biochem. J.62, 315–322 (1956).
Chhibber S., Bajaj J.: Polysaccharide-iron regulated cell surface protein conjugate vaccine: its role in protection againstKlebsiella pneumoniœ induced lobar pneumonia.Vaccine13, 179–184 (1995).
Cryz S.J., Jr,Furer E., Germanier F.R.: Protection against Klebsiella pneumoniae burn wound sepsis by passive transfer of anticapsular polysaccharide.Infect. Immun.45, 139–142 (1984).
Cryz S.J. Jr.,Furer E., Germanier F.R.: Safety and immunogenicity ofKlebsiella pneumoniœ K1 capsular polysaccharide vaccine in humans.J. Infect. Dis.151, 665–671 (1985).
Cryz S.J. Jr.,Mortimer P.M., Mausfield V., Germanier R.: Seroepidemiology ofKlebsiella pneumoniœ bacteremic isolates and implications for vaccine development.J. Clin. Microbiol.23, 687–690 (1986a).
Cryz S.J. Jr.,Furer E., Germanider R.: Safety and immunogenicity of a polyvalentKlebsiella capsular polysaccharide vaccine in humans.Vaccine4, 15–20 (1986b).
Galelli A., Parant M., Chedid L.: Role of radiosensitive and radioresistant cells in nonspecific resistance to infection of LPS-treated mice.J. Reticulo-endothel. Soc.21, 109–118 (1977).
Hoffman N.R., Preston F.S.: Friedlander's pneumonia.Dis. Chest.53, 481–486 (1968).
Jarvis W.R., Munn V.P., Highsmith A.K., Culvar D.H., Hughs J.M.: The epidemiology of nosocomial infections caused byKlebsiella pneumoniœ.Infect Control (Thorofares)6, 68–74 (1985).
Johnson K.G., Perry M.B.: Improved techniques for the preparation of bacterial lipopolysaccharides.Can. J. Microbiol.22, 29–34 (1976).
Kapoor N., Chhibber S., Vadehra D.V.: Sensitivity of multidrug resistantKlebsiella pneumoniœ to silver nitrate.Folia Microbiol.34, 94–98 (1989).
Lasfargues A., Ledur A., Charon, Szabo L., Chaby R.: Induction by lipopolysaccharide of intracellular and extracellular interleukin-1 production. Analysis with synthetic models.J. Immunol.139, 429–436 (1987).
Mizuta K., Ohta M., Mori M., Hasegawa T., Nakashima N.T., Kato N.: Virulence for mice ofKlebsiella strains belonging to the O1-group: Relationship to their capsular (k) types.Infect. Immun.40, 56–61 (1983).
Morrison D.C., Leive L.: Fractions of lipopolysaccharide fromEscherichia coli O111: B4 prepared by two extraction procedures.J. Biol. Chem.250, 2911–2919 (1975).
Munro H.N., Fleck A.: The determination of nucleic acids.Methods Biochem. Anal.14, 113–176 (1966).
Rani M., Gupta R.K., Chhibber S.: Protection againstKlebsiella pneumoniœ induced lobar pneumonia in rats with lipopolysaccharide and related antigens.Can. J. Microbiol.36, 885–890 (1990).
Rietschel E.T., Schade V., Jensen M., Wollenweber H.W., Luderitz O., Greisman S.E.: Bacterial endotoxins: Chemical structure, biological activity and role in septicemia.Scand. J. Infect. Dis.37, 8–21 (1982).
Sinha N., Harjal K., Sharma S.: Ascending pyelonephritis model in Swiss Webster (LACA) mice.Ind. J. Med. Res.88, 541–544 (1988).
Vuopio-Varkila J., Nurminen M., Phyhala L., Makela P.H.: Lipopolysaccharide induced nonspecific resistance to systemicEscherichia coli infection in mice.J. Med. Microbiol.25, 197–204 (1988).
Westphal O., Jann K.: Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure, pp. 83–91 inMethods in Carbohydrate Chemistry, Vol. 5 (R.L. Whistler, Ed.). Academic Press, New York 1965.
Williams P., Lambert P.A., Brown M.R.W.: Penetration of immunoglobulins throughKlebsiella capsule and their effect of cell-surface hydrophobicity.J. Med. Microbiol.26, 29–36 (1988).
Wright S.D., Jong M.T.C.: Adhesion-promoting receptors on human macrophages recognizeEscherichia coli by binding to lipopolysaccharide.J. Exp. Med.164, 1876–1888 (1986).
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Chopra Nee Chathley, U., Sharma, S. & Chhibber, S. Lipopolysaccharide-induced resistance in mice against ascending urinary tract infection withklebsiella pneumoniœ . Folia Microbiol 41, 373–376 (1996). https://doi.org/10.1007/BF02814718
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DOI: https://doi.org/10.1007/BF02814718