Abstract
Using simultaneous fusion of protoplasts of three strains the auxotrophic K3 killer designated MH1 was prepared at a very low frequency and further employed for the transfer of the K3 killer character into a commercial wine yeast. A novelSaccharomyces Bratislava 1K3 strain with desirable technological and anti-yeast killer abilities was thus constructed. Attempts to prepare double K1/K3 killers were made. The obtained fusion products contained M1 dsRNA and were able to produce only the K1 type killer toxin.
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Sulo, P., Michalčáková, S. The K3 type killer strains of genusSaccharomyces for wine production. Folia Microbiol 37, 289–294 (1992). https://doi.org/10.1007/BF02814566
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DOI: https://doi.org/10.1007/BF02814566