Summary
When protoplasts isolated from non-respiring rho 0 strains of S. cerevisiae are incubated with mitochondria from respiring rho + strains in the presence of polyethylene glycol, a small fraction of the acceptor protoplasts acquires respiratory capacity. Pretreatment of the isolated mitochondria and protoplasts with Ca2+ ions enhanced the frequency of ensuing respiration-competent colonies by almost three orders of magnitude. When mitochondria were isolated from a strain exhibiting killer capacity as a cytoplasmic marker, in addition to the appropriate nuclear and mitochondrial markers, about one-third of the ensuing rho + colonies were not killers. Possible mechanisms for the transfer of mitochondria into acceptor protoplasts were studied.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Birnboim HC, Doly J (1979) Nucleic Acid Res 7:1513–1523
Boynton JE, Gillham NW, Harris EH, Hosler JP, Johnson AM, Jones AR, Randolph-Anderson BL, Robertson D, Klein TM, Shark KB, Sanford JC (1988) Science 240:1534–1538
Curran BPG, Carter BLA (1986) Curr Genet 10:943–945
El-Sherbeini M, Bostian KA (1987) Proc Natl Acad Sci USA 84:4293–4297
Fink GR, Styles GN (1972) Proc Natl Acad Sci USA 69:2846–2849
Fried HM, Fink GR (1978) Proc Natl Acad Sci USA 75:4224–4228
Fukuda H, Kimura A (1980) FEBS Lett 113:58–60
Goodey AR, Bevan EA (1983) Curr Genet 7:69–72
Gunge N, Sakaguchi K (1979) Mol Gen Genet 170:243–247
Hrmová M, Farkaš V, Kopecká M (1984) J Microbiol Methods 2:257–260
Johnston SA, Anziano PQ, Shark K, Sanford JC, Butow RA (1988) Science 240:1538–1541
Kopecká M, Gabriel M, Nečas O (1974) J Gen Microbiol 81:111–120
Kováč L, Bednárová H, Greksák M (1968) Biochim Biophys Acta 153:32–42
Lang B, Burger G, Doxiadis I, Thomas DY, Bandlow W, Kaudewitz F (1977) Anal Biochem 77:110–121
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) J Biol Chem 193:265–275
Ortega Ruiz JM (1977) Microbios Lett 4:133–137
Rose MD, Price BR, Fink GR (1986) Mol Cell Biol 6:3490–3497
Tipper DJ, Bostian KA (1984) Microbiol Rev 48:126–156
Tuppy H, Wildner G (1965) Biochem Biophys Res Commun 20:733–738
Wickner RB (1983) Arch Biochem Biophys 222:1–11
Wickner RB (1986) Annu Rev Biochem 55:373–395
Yoshida K (1979) Plant Cell Physiol 20:851–856
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sulo, P., Griač, P., Klobučníková, V. et al. A method for the efficient transfer of isolated mitochondria into yeast protoplasts. Curr Genet 15, 1–6 (1989). https://doi.org/10.1007/BF00445745
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00445745