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Inoculation ofRhododendron cv. Belle-Heller with two strains ofPhialocephala fortinii in two different substrates

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Abstract

The growth response of an ornamentalRhododendron hybrid to the inoculation withPhialocephala fortinii was studied in two pot experiments in order to decide about the effectiveness of the inoculation of young rhododendron microplants. Two different substrates were used in both experiments, either sterilized or non-sterilized: a horticultural substrate and a soil collected from a field site with dominant ericoid vegetation. Two fungal isolates were used for an inoculation:P. fortinii strain P (UAMH 8433) andP. fortinii strain F, a dark septate endophyte (DSE) previously isolated from naturally-infected roots ofVaccinium myrtillus. BothPhialocephala strains successfully colonized the roots of the host plants forming typical DSE (=pseudomycorrhizal) colonization pattern including the formation of intracellular microsclerotia. However, pseudomycorrhizal colonization did not affect the growth parameters of the host rhododendrons. The results from both experiments indicate a neutral effect of the inoculation with DSE fungi on the growth ofRhododendron cv. Belle-Heller.

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References

  • Bajwa R. &Read D.J. (1986): Utilization of mineral and amino N sources by the ericoid mycorrhizal endophyteHymenoscyphus ericae and by mycorrhizal and non-mycorrhizal seedlings ofVaccinium.Trans. Brit. Mycol. Soc. 87: 269–277.

    Article  CAS  Google Scholar 

  • Barrow J.R. &Aaltonen R.E. (2001): Evaluation of the internal colonization ofAtriplex canescens (Pursh)Nutt. roots by dark septate fungi and influence of host physiological activity.Mycorrhiza 11: 199–205.

    Article  Google Scholar 

  • Currah R.S., Tsuneda A. &Murakami S. (1993): Morphology and ecology ofPhialocephala fortinii in roots ofRhododendron brachycarpum.Canad. J. Bot. 71: 1639–1644.

    Google Scholar 

  • Currah R.S. &Tsuneda A. (1993): Vegetative and reproductive morphology ofPhialocephala fortinii (Hyphomycetes, Mycelium radicis atrovirens) in culture.Trans. Mycol. Soc. Japan 34: 345–356.

    Google Scholar 

  • Fernando A.A. &Currah R.S. (1996): A comparative study of the effects of the root endophytesLeptodontidium orchidicola andPhialocephala fortinii (Fungi Imperfecti) on the growth of some subalpine plants in culture.Canad. J. Bot. 74: 1071–1078.

    Google Scholar 

  • Hambleton S. &Currah R. S. (1997): Fungal endophytes from the roots of alpine and borealEricaceae.Canad. J. Bot. 75: 1570–1581.

    Article  Google Scholar 

  • Haselwandter K. &Read D.J. (1980): Fungal associations of roots of dominant and sub-dominant plants in high-alpine vegetation systems with special reference to mycorrhiza.Oecologia 45: 57–62.

    Article  Google Scholar 

  • Jansa J. &Vosátka M. (2000): In vitro and post vitro inoculation of micropropagated rhododendrons with ericoid mycorrhizal fungi.Appl. Soil Ecol. 15: 125–136.

    Article  Google Scholar 

  • Jumpponen A. (2001): Dark septate endophytes — are they mycorrhizal?Mycorrhiza 11: 207–211.

    Article  Google Scholar 

  • Jumpponen A., Mattson K.G. &Trappe J.M. (1998): Mycorrhizal functioning ofPhialocephala fortinii withPinus contorta on glacier forefront soil: interactions with soil nitrogen and organic matter.Mycorrhiza 7: 261–265.

    Article  CAS  Google Scholar 

  • Jumpponen A. &Trappe J.M. (1998a): Dark septate endophytes: a review of facultative biotrophic root — colonizing fungi.New Phytol. 140: 295–310.

    Article  Google Scholar 

  • Jumpponen A. &Trappe M.J. (1998b): Performance ofPinus contorta inoculated with two strains of root endophytic fungus,Phialocephala fortinii: effects of synthesis system and glucose concentration.Canad. J. Bot. 76: 1205–1213.

    Article  CAS  Google Scholar 

  • Lemoine M.C., Gianinazzi S. &Gianinazzi-Pearson V. (1992): Application of endomycorrhizae to commercial production ofRhododendron microplants.Agronomie 12: 881–885.

    Article  Google Scholar 

  • Litterick A.M., McQuilken M.P. &Holmes S.J. (1995): Sources ofRhizoctonia species in ericaceous plant nurseries.J. Pl. Dis. Protect. 102: 441–444.

    Google Scholar 

  • McLean C.B., Cunnington J.H. &Lawrie A.C. (1999): Molecular diversity within and between ericoid endophytes from theEricaceae andEpacridaceae.New Phytol. 144: 351–358.

    Article  CAS  Google Scholar 

  • Monreal M., Berch S. M. &Berbee M. (1999): Molecular diversity of ericoid mycorrhizal fungi.Canad. J. Bot. 77: 1580–1594.

    Article  CAS  Google Scholar 

  • Moore-Parkhurst S. &Englander L. (1982): Mycorrhizal status ofRhododendron spp. in commercial nurseries in Rhode Island.Canad. J. Bot. 60: 2342–2344.

    Google Scholar 

  • O’Dell T.E., Massicotte H.B. &Trappe J.M. (1993): Root colonization ofLupinus latifolius Agardh. andPinus contorta Dougl. byPhialocephala fortinii Wang & Wilcox.New Phytol. 124: 93–100.

    Article  Google Scholar 

  • Phillips J.M. &Hayman S.S. (1970): Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection.Trans. Brit. Mycol. Soc. 55: 158–160.

    Article  Google Scholar 

  • Read D.J. &Haselwandter K. (1981): Observations on the mycorrhizal status of some alpine plant communities.New Phytol. 88: 341–352.

    Article  Google Scholar 

  • Sharples J.M., Chambers S.M., Meharg A.A. &Cairney J.W.G. (2000): Genetic diversity of root-associated fungal endophytes fromCalluna vulgaris at contrasting field sites.New Phytol. 148: 153–162.

    Article  CAS  Google Scholar 

  • Starrett M.C., Blazich F.A., Shafer S.R. &Grand L.F. (2001): In vitro colonization of micropropagatedPieris floribunda by ericoid mycorrhizae. I. Establishment of mycorrhizae on microshoots.Hortscience 36: 353–356.

    CAS  Google Scholar 

  • Stoyke G. &Currah R.S. (1993): Resynthesis in pure culture of a common subalpine fungus-root association usingPhialocephala fortinii andMenziesia ferruginea (Ericaceae).Arctic Alpine Res. 25: 189–193.

    Article  Google Scholar 

  • Straker C.J. (1996): Ericoid mycorrhiza: ecological and host specificity.Mycorrhiza 6: 215–225.

    Article  Google Scholar 

  • Vodnik D., Mihelčič M. &Gogala N. (1997): Isolation of root associated fungi fromErica herbacea and their tolerance to lead.Acta Biol. Slovenica 41(4): 35–42.

    Google Scholar 

  • Vohník M. (2002):Inoculation of Vaccinium vitis-idaeaand Rhododendroncv. Belle-Heller with ericoid mycorrhizal Oidiodendron griseumand pseudomycorrhizal Phialocephala fortinii. MSc. Thesis. Department of Plant Physiology, Faculty of Science, Charles University, Prague.

    Google Scholar 

  • Wang C.J.K. &Wilcox H.E. (1985): New species of ectendomycorrhizal and pseudomycorrhizal fungi:Phialophora finlandia, Chloridium paucisporum andPhialocephala fortinii.Mycologia 77: 951–958.

    Article  Google Scholar 

  • Wilcox H.E. &Wang C.J.K. (1987): Mycorrhizal and pathological associations of dematiaceous fungi in roots of 7-month-old tree seedlings.Canad. J. Forest Res. 17: 884–899.

    Google Scholar 

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Vohník, M., Lukančič, S., Bahor, E. et al. Inoculation ofRhododendron cv. Belle-Heller with two strains ofPhialocephala fortinii in two different substrates. Folia Geobot 38, 191–200 (2003). https://doi.org/10.1007/BF02803151

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