Skip to main content
SpringerLink
Log in

Source, alterations, characteristics and use of a new dog cell line (Cf2Th)

  • Published:
In Vitro Aims and scope Submit manuscript

Summary

A cell line derived from normal fetal canine thymus (Cf2Th) has been in culture since 1967. During cultivation the cells have changed morphologically from a fibroblast-like to flat, fusiform appearance and karyologically from diploid (2n=78) with 76 telocentric autosomes to hypodiploid with newly formed atelocentric chromosomes. The cells retain canine characteristic enzyme activity (G6PD and LDH) as well as cell membrane fluorescence and are free of mycoplasma. High passage cells produce tumors in ATST mice. No endogenous viruses have been detected in these cells. No original publication exists, to date, on the origin of this line, but seed stocks thereof have been distributed to many laboratories and the cells have served as experimental substrates in a number of published works in oncology albeit under different designations. The present information is offered in order to establish the provenance of this valuable cell line and to list characteristics which may serve to monitor for its purity and to distinguish it from other existing cell lines of dog origin also in common use.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Todaro, G. J., R. E. Benveniste, J. M. Lieber, and D. M. Livingston. 1973. Infectious type C viruses released by normal cat embryo cells. Virology 55: 506–515.

    Article  PubMed  CAS  Google Scholar 

  2. Benveniste, R. E., M. M. Lieber, D. M. Livingston, C. J. Sherr, G. J. Todaro, and S. S. Kalter, 1974. Infectious C-type, virus isolated from a baboon placenta. Nature 248: 17–20.

    Article  PubMed  CAS  Google Scholar 

  3. Todaro, G. J., C. J. Sherr, R. E. Benveniste, M. M. Lieber, and J. L. Melnick. 1974. Type C viruses of baboons: isolation from normal cell cultures. Cell 2: 55–61.

    Article  PubMed  CAS  Google Scholar 

  4. Geldberg, R. J., E. M. Scolnick, W. P. Parks, L. A. Yakovleva, and B. A. Lapin. 1974. Isolation of primate type-C virus from a lymphomatous baboon. Int. J. Cancer 14: 722–730.

    Article  Google Scholar 

  5. Teich, N. M., R. A. Weiss, S. A. Zalahuddin, R. E. Gallagher, D. H. Gillespie, and R. C. Gallo. 1975. Infective transmission and characterization of a C-type virus released by cultured human myeloid leukemia cells. Nature 256: 551–555.

    Article  PubMed  CAS  Google Scholar 

  6. Lieber, M. M., C. J. Sherr, and G. J. Todaro. 1974. S-tropic murine type-C viruses: frequency of isolation from continuous cell lines, leukemia virus preparations and normal spleens. Int. J. Cancer 13: 587–598.

    Article  PubMed  CAS  Google Scholar 

  7. Arnstein, P., D. O. N. Taylor, W. A. Nelson-Rees, R. J. Huebner, and E. H. Lennette. 1974. Propagation of human tumors in antithymocyte serum-treated mice. J. Natl. Cancer Inst. 52: 71–84.

    PubMed  CAS  Google Scholar 

  8. Robertson, W. R. B. 1916. Chromosome studies. J. Morphol. 27: 179–331.

    Article  Google Scholar 

  9. Nelson-Rees, W. A., A. J. Kniazeff, and N. B. Darby, Jr. 1967. Debut and accumulation of centric fusion products: an index to age of certain cell lines. Cytogenetics 6: 436–450.

    PubMed  CAS  Google Scholar 

  10. Miles, C. P., G. Moldavannu, D. G. Miller, and A. Moore. 1970. Chromosome analysis of canine lymphosarcoma: two cases involving probable centric fusion. Am. J. Vet. Res. 31: 783–790.

    PubMed  CAS  Google Scholar 

  11. Shannon, J. E. (Ed.).The American Type Culture Collection: Registry of Animal Cell Lines. Second ed. American Type Culture Collection, Rockville, Md.

  12. Gaush, C. R., W. L. Hard, and T. F. Smith. 1969. Characterization of an established line of canine kidney cells. Proc. Soc. Exp. Biol. Med. 122: 931–935.

    Google Scholar 

  13. Riggs, J. L., R. M. McAllister, and E. H. Lennette. 1974. Immunofluorescent studies of RD-114 virus replication in cell culture. J. Gen. Virol. 25: 21–29.

    Article  PubMed  CAS  Google Scholar 

  14. Arnstein, P., A. Levy, L. S. Oshiro, P. J. Price, W. Suk, and E. H. Lennette. 1974. Recovery of murine xenotropic type-C virus from C57L mice. J. Natl. Cancer Inst. 53: 1787–1791.

    PubMed  CAS  Google Scholar 

  15. Jensen, E. M., E. E. Force, and J. B. Unger. 1961. Inhibitory effect of ammonium ions on influenza virus in tissue culture. Proc. Soc. Exp. Biol. Med. 107: 447–451.

    PubMed  CAS  Google Scholar 

  16. Jensen, E. M., and O. C. Liu. 1963. Inhibitory effect of simple aliphatic amines on influenza virus in tissue culture. Proc. Soc. Exp. Biol. Med. 112: 456–459.

    CAS  Google Scholar 

  17. Leighton, J., Z. Brada, L. W. Estes, and G. Justh. 1969. Secretory activity and oncogenicity of a cell line (MDCK) derived from canine kidney. Science 163: 472–473.

    Article  PubMed  CAS  Google Scholar 

  18. Brown, A. L., E. V. Davis, and W. H. Beckenhover. 1968. Studies of an established canine kidney-cell line. Cornell Vet. 58: 593–613.

    Google Scholar 

  19. Oie, H. K., E. K. Russell, J. H. Dotson, J. M. Rhoads, and A. F. Gazdar. 1976. Host-Range properties of murine xenotropic and ecotropic type C. J. Natl. Cancer Inst. 56: 423–426.

    PubMed  CAS  Google Scholar 

  20. Oshimura, M., M. Sasaki, and S. Makino. 1973. Chromosomal banding patterns in primary and transplanted veneral tumors of the Dog. J. Natl. Cancer Inst. 51: 1197–1203.

    PubMed  CAS  Google Scholar 

  21. Selden, J. R., P. S. Moorhead, M. L. Oehlert and D. F. Patterson. 1975. The giemsa banding pattern of the canine karyotype. Cytogenet. Cell Genet. 15: 380–387.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cell Culture Laboratory, University of California, School of Public Health, Naval Biomedical Research Laboratory, 94625, Oakland, Calif.

    W. A. Nelson-Rees & R. B. Owens

  2. Viral Carcinogenesis Branch, National Cancer Institute, National Institutes of Health, Public Health Service, U. S. Department of Health, Education and Welfare, 20014, Bethesda, Md.

    P. Arnstein

  3. University of California Resources, 92037, La Jolla, Calif.

    A. J. Kniazeff

Authors
  1. W. A. Nelson-Rees
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. B. Owens
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Arnstein
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. J. Kniazeff
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by Contract No. 1-CP-3-3237 within the Virus Cancer Program, National Cancer Institute, National Institutes of Health.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nelson-Rees, W.A., Owens, R.B., Arnstein, P. et al. Source, alterations, characteristics and use of a new dog cell line (Cf2Th). In Vitro Cell.Dev.Biol.-Plant 12, 665–669 (1976). https://doi.org/10.1007/BF02797468

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02797468

Key words

Search

Navigation