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Isolation and Characterization of Cancer Stem Cells from Dog Glioblastoma

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Stem Cells and Cancer Stem Cells, Volume 10

Part of the book series: Stem Cells and Cancer Stem Cells ((STEM,volume 10))

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Abstract

There is increasing evidence in some malignancies that the tumor clone is heterogeneous (phenotypically and functionally) in regard to proliferation and differentiation. The cancer stem cell hypothesis implies that not all the cells in the tumor have the same capacity to proliferate and maintain the growth of the tumor. Only a relative small fraction of cells in the tumor, termed cancer stem cells (CSCs), possess the ability to proliferate and self-renew extensively. In the past decade, several groups have reported the existence of a CSC population in different human brain tumors from both children and adults. Our study demonstrated the presence of a CSC population from a dog with glioblastoma multiforme (GBM) that possesses a great capacity for proliferation, self-renewal, and differentiation. This cell line is aneuploid, forms neurospheres in culture, possesses CSC markers, and reproduces the original dog GBM when inoculated into the nude mouse brain. A comparative approach to the study of canine glioblastoma CSC provides an excellent animal model for human glioblastoma pathogenesis and development of novel therapies in both species.

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Acknowledgments

This work was supported in part by a grant from Canine Health Foundation (Acorn), USA.

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Authors and Affiliations

  1. Veterinary Pathobiology, Texas A&M University, College Station, TX, 77843-4467, USA

    George Stoica & Gina Lungu

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  1. George Stoica
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  2. Gina Lungu
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Correspondence to George Stoica .

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Editors and Affiliations

  1. , Kean University, Room 213, Library building, Morris Avenue 1000, Union, 07083, New Jersey, USA

    M.A. Hayat

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Stoica, G., Lungu, G. (2013). Isolation and Characterization of Cancer Stem Cells from Dog Glioblastoma. In: Hayat, M. (eds) Stem Cells and Cancer Stem Cells, Volume 10. Stem Cells and Cancer Stem Cells, vol 10. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-6262-6_20

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