Summary
Protein content, dry weight, cell viability, and the ability of cultures to be subcultured were measured at 4-d intervals beginning on Day 14 when cell division and expansion had stopped. Measurements were continued until the cultures had died.
The protein content of senescing cultures decreased from approximately 780 to 500 µg between Days 14 and 18, then remained relatively constant until Day 26 whereupon it declined to approximately 350 µg/culture. The decline in protein content of parent cultures did not correlate well with cell viability and their ability to be subcultured at some ages.
In older cultures it was estimated that less than 2000 of the approximately 261×106 cells appeared to be capable of dividing when they were subcultured.
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This work was supported by National Institute of Health Grant 1 R01 AG01709-02.
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Reider, M.L., Smith, B.A. & Fletcher, J.S. Protein content and subculturing properties of senescing plant suspension cultures. In Vitro Cell.Dev.Biol.-Plant 18, 1004–1008 (1982). https://doi.org/10.1007/BF02796375
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DOI: https://doi.org/10.1007/BF02796375