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Bacitracin production by a new strain ofBacillus subtilis

Extraction, purification, and characterization

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Abstract

A new strain ofBacillus subtilis C 126 was isolated from sugar cane fermentation and produced an antibiotic that inhibited the growth ofMicrococcus flavus. The production of the antibiotic in culture medium followed to extraction withn-butanol, thin layer chromatography, and microbiological tests indicated that a polypeptide antibiotic was produced. The fraction obtained by Sephadex G-25 column and analyzed by HPLC indicated that bacitracin complex was produced.

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Authors and Affiliations

  1. Mestrado de Ciencias FarmacÊuticas, Universidade Federal de Pernambuco, 50739, Recife-PE, Brazil

    E. C. Azevedo

  2. Depto. de Antibióticos, Universidade Federal de Pernambuco, 50739, Recife-PE, Brazil

    E. M. Rios & G. M. Campos-Takaki

  3. Laboratório de Imunopatologia Prof. Keizo Asami-LIKA, Universidade Federal de Pernambuco, 50739, Recife-PE, Brazil

    K. Fukushima & G. M. Campos-Takaki

Authors
  1. E. C. Azevedo
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  2. E. M. Rios
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  3. K. Fukushima
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  4. G. M. Campos-Takaki
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Azevedo, E.C., Rios, E.M., Fukushima, K. et al. Bacitracin production by a new strain ofBacillus subtilis . Appl Biochem Biotechnol 42, 1–7 (1993). https://doi.org/10.1007/BF02788897

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  • DOI: https://doi.org/10.1007/BF02788897

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