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Detection of plant genes, gene expression and viral RNA from tissue prints on FTA cards

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Abstract

A procedure was established for easy and convenient detection of plant DNA, plant RNA and viral RNA from plant tissue prints on FTA® (Flinders Technology Associates, Moscoso et al., 2005) PlantSaver Cards, while avoiding the cross-contamination that commonly occurs with prints from plant tissues. Detection was successful by adding 2 mm discs of the prints directly to (RT)-PCR reactions. DNA was detected in leaves from tobacco, tomato and grapes, and in fruit of tomato and grape. Rubisco and malate dehydro-genase RNA were detected in tomato fruit and leaf, tobacco leaf and grape leaf. RNA from Pepino mosaic virus was detected in tomato leaf and fruit and from Rupestis stem pitting associated virus in grape leaf. Detection was still possible from the tissue prints on FTA® Cards after storage for many months at room temperature, making this a procedure suitable for sample collection and storage off site, prior to further processing in the laboratory.

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Abbreviations

DTT:

dithiothreitol

FTA:

Flinders Technology Associates

PepMV:

pepino mosaic virus

RSPaV:

Rupestis stem pitting-associated virus

RT-PCR:

reverse transcription-polymerase chain reaction

RubiscoL:

large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase

SSR:

simple sequence repeat

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Authors and Affiliations

  1. Department of Molecular and Cellular Biology, University of Guelph, N1G 2W1, Guelph, ON, Canada

    Yvette Roy & Annette Nassuth

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  1. Yvette Roy
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  2. Annette Nassuth
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Correspondence to Annette Nassuth.

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Roy, Y., Nassuth, A. Detection of plant genes, gene expression and viral RNA from tissue prints on FTA cards. Plant Mol Biol Rep 23, 383–395 (2005). https://doi.org/10.1007/BF02788886

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