Abstract
The deposit of DNA samples of wild plants that correspond to voucher specimens is highly informative and greatly enhances the value of the herbarium specimens. The Society of Himalayan Botany (SHB), Tokyo, has assembled general collections of flowering plants of the Sino-Himalayan region for more than 40 years. In a trial of the collection of these types of bioresources for use in basic research, we adopted FTA cards, which have recently been used for large-scale collection of DNA of humans, microorganisms and viruses, for the general collection of DNA samples of wild plants during a botanical expedition in Mustang, Nepal, in 2003. Three hundred and fifty-five plant specimens from Mustang, Nepal, were collected along with the corresponding DNA samples. Examination of the quality of the DNA samples by PCR demonstrated the utility of the collection system. The identification of all of the specimens collected, as well as data from the specimens, will be presented on the Flora of Nepal Database website (http://ti.um.u-tokyo.ac.jp/default.htm), which is open to the public. The DNA resources will be identified on the website and distributed openly by the SHB to researchers worldwide for basic research.
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Acknowledgements
The authors wish to give special thanks to the following colleagues who assisted in the botanical expedition in the field: Dr. T. Miyazaki (University of Tokyo, Japan), Dr. K. Yonekura (Tohoku University, Japan), Dr. Y. Ibaragi (Tokushima Prefectural Museum, Japan), and Dr. R.K. Uprety (Ministry of Forest and Soil Conservation, Nepal). The Mustang botanical expedition was carried out as a cooperative project between the Department of Plant Resources, Nepal, and the Department of Botany, University Museum, University of Tokyo, Japan. This study was supported by a grant from the Midori-ikusei-Zaidan, the SOKENDAI group research project from the Graduate University for Advanced Studies, and a Grant-in-Aid from the Ministry of Education, Science, and Culture, Japan and the Japan Society for the Promotion of Science.
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Tsukaya, H., Iokawa, Y., Kondo, M. et al. Large-scale general collection of wild-plant DNA in Mustang, Nepal. J Plant Res 118, 57–60 (2005). https://doi.org/10.1007/s10265-005-0196-4
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DOI: https://doi.org/10.1007/s10265-005-0196-4