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Design of a spray-cycle bioreactor and its application for riboflavin production

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Abstract

A highly efficient spray-cycle reactor for oxygen supply was developed. A typical arrangement of the reactor consists of a spray column fitted with a nozzle and a coaxal tube, and a reservoir vessel. The culture broth was circulated between the column and vessel by a peristaltic pump. The volumetric oxygen-transfer coefficient, k1a was evaluated as a parameter for oxygen supply. The liquid circulation rate in the spraycycle reactor was represented in terms of the number of circulations. The k1a value increased as the number of circulations increased, reaching 208 h-1 at 4.4 min-1 of circulation numbers. This value was 1.8 times higher than that in a 1500-mL stirred-tank reactor under the agitation of 20.7g and the aeration of 1.0 volume per min.

The spray-cycle reactor was applied to riboflavin production by an aerobic microorganism. The riboflavin production increased as k1a values increased and the maximal riboflavin production was 161 mg/L at 208 h-1 of k1a. These results suggest that the spray-cycle reactor is useful to oxygen-demanding fermentation because of the high k1a value in comparison with the stirred-tank reactor.

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Authors and Affiliations

  1. Department of Bioapplied Chemistry, Osaka City University, Sugimoto, Sumiyoshi-ku, 558-8585, Osaka, Japan

    Hlroshi Ooshima, Yoshiharu Yamane, Yozo Nakamura, Hideki Sakashita, Masayuki Azuma & Jyoji Kato

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  1. Hlroshi Ooshima
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  2. Yoshiharu Yamane
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  3. Yozo Nakamura
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  4. Hideki Sakashita
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  5. Masayuki Azuma
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  6. Jyoji Kato
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Corresponding author

Correspondence to Jyoji Kato.

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Ooshima, H., Yamane, Y., Nakamura, Y. et al. Design of a spray-cycle bioreactor and its application for riboflavin production. Appl Biochem Biotechnol 73, 51–58 (1998). https://doi.org/10.1007/BF02788833

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  • DOI: https://doi.org/10.1007/BF02788833

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