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Electrokinetic response of granulocytes to concanavalin A and succinyl-concanavalin A

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Abstract

Electrophoretic light scattering has been used to study the effects of concavalin A (Con A) and succinyl-Con A on the electrophoretic mobility distribution of resident guinea-pig peritoneal eosinophils and human peripheral blood polymorphonuclear leukocytes. In both cell types, incubation with Con A (a tetrameric lectin) decreases slightly the mean mobility and increases substantially the width of the electrophoretic mobility distribution. These effects can be abolished by α-methyl-D-mannoside, a hapten sugar of Con A. Succinyl Con A, a dimeric derivative, was found to have no effect on the mobility distribution. These results are strikingly similar to our previous report of the response of the resident guinea-pig macrophage (19), suggesting possible parallels in the endocytic mechanisms of these cell types.

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Author notes

  1. Howard R. Petty

    Present address: Stauffer Laboratory for Physical Chemistry, Stanford University, 94305, Stanford, California

  2. B. R. Ware

    Present address: Department of Chemistry, Syracuse University, 13210, Syracuse, New York

Authors and Affiliations

  1. Department of Chemistry, Harvard University, 02138, Cambridge, Massachusetts

    Howard R. Petty & B. R. Ware

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  1. Howard R. Petty
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  2. B. R. Ware
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Petty, H.R., Ware, B.R. Electrokinetic response of granulocytes to concanavalin A and succinyl-concanavalin A. Cell Biochem Biophys 3, 19–28 (1981). https://doi.org/10.1007/BF02782150

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  • DOI: https://doi.org/10.1007/BF02782150

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