Skip to main content
SpringerLink
Log in

Electron microscopical studies on the structure, phagocytic properties, and peroxidatic activity of resident and exudate peritoneal macrophages in the guinea pig

  • Published:
Zeitschrift für Zellforschung und Mikroskopische Anatomie Aims and scope Submit manuscript

Summary

A comparative study on the fine structure, phagocytic properties, and peroxidatic activity of guinea pig resident peritoneal macrophages and the monocytes and macrophages present in experimentally induced sterile peritoneal exudates is reported. A number of fine-structural characteristics of the resident macrophages are described, including a peculiar configuration of the cell surface termed “loops”. The structure of these loops suggests the existence of a cell coat on the cytoplasmic side of the plasma membrane.

The following observations were also made. Exudate monocytes differ from resident peritoneal macrophages mainly by the limitation of peroxidatic activity to cytoplasmic granules. The resident peritoneal macrophages have peroxidatic activity in the endoplasmic reticulum, the nuclear envelope, the annulate lamellae, and some of the cisternae of the Golgi apparatus. Macrophages derived from exudate monocytes do not show the same distribution of peroxidatic activity as the resident peritoneal macrophages. Both types of macrophages are highly active in the uptake of exogenous material. The functional significance of these observations is discussed and the conclusion is drawn that peritoneal exudates contain two different types of macrophages: resident macrophages and exudate macrophages.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ackerman, G.A.: Structural studies of the lymphocyte and lymphocyte development. In: Regulation of hematopoiesis, vol. 2, White cell and platelet production (A.S. Gordon, ed.), p. 1297–1377. New York: Appleton-Century-Crofts 1970

    Google Scholar 

  • Allison, A.C., Davies, P., Petris, S. de: Endocytosis: Role of contractile microfilaments in macrophages movement and endocytosis. Nature (Lond.) New. Biol. 232, 153–155 (1971)

    Google Scholar 

  • Aronow, R., Danon, D., Shahar, A., Aronson, M.: Electron microscopy of in vitro endocytosis of T2 phage by cells from rabbit peritoneal exudate. J. exp. Med. 120, 943–954 (1964)

    Google Scholar 

  • Athanassiades, T.J., Speirs, R.S.: Granuloma induction in the peritoneal cavity. A model for the study of inflammation and plasmacytopoiesis in nonlymphatic organs. J. reticuloendoth. Soc. 11, 60–76 (1972)

    Google Scholar 

  • Atwal, O.S.: Cytoenzymological behavior of peritoneal exudate cells of rat in vivo. I. Histochemical study of enzymatic function of peroxidase. J. reticuloendoth. Soc. 10, 163–172 (1971)

    Google Scholar 

  • Axline, S.G.: Functional biochemistry of the macrophage. Sem. Haematol. 7, 142–160 (1970)

    Google Scholar 

  • Baggiolini, M., Hirsch, J.G., de Duve, C.: Resolution of granules from rabbit heterophil leukocytes into distinct populations by zonal sedimentation. J. Cell Biol. 40, 529–541 (1969)

    Google Scholar 

  • Baggiolini, M., Hirsch, J.G., de Duve, C.: Further biochemical and morphological studies of granule fractions from rabbit heterophil leukocytes. J. Cell Biol. 45, 586–597 (1970)

    Google Scholar 

  • Bennett, B., Old, L.J., Boyce, E.A.: The phagocytosis of tumor cells in vitro. Transplantation 2, 183–202 (1964)

    Google Scholar 

  • Bhisey, A.N., Freed, J.J.: Ameboid movement induced in cultured macrophages by colchicine or vinblastine. Exp. Cell Res. 64, 419–429 (1971)

    Google Scholar 

  • Bowden, D.H., Adamson, I.Y.R.: The pulmonary interstitial cell as immediate precursor of the alveolar macrophage. Amer. J. Path. 68, 521–528 (1972)

    Google Scholar 

  • Bowden, D.H., Adamson, I.Y.R., Grantham, W.G., Wyatt, J.P.: Origin of the lung macrophage. Arch. Path. 88, 540–546 (1969)

    Google Scholar 

  • Braunsteiner, H., Schmalzl, F.: Cytochemistry of monocytes and macrophages. In: Mononuclear phagocytes (R. van Furth, ed.), p. 62–81. Oxford-Edinburgh: Blackwell Sci. Publ. 1970

    Google Scholar 

  • Brederoo, P., Daems, W.Th.: Submicroscopic cytology of guinea pig peritoneal exudates. I. The heterogeneity of the granules of neutrophilic granulocytes. In: Microscopie électronique 1970 (P. Favard, ed.). Rés. 7e Congr. Intern. Grenoble 1970. Vol. III, p. 541–542. Paris: Soc. Franç. Microsc. Electr. 1970

    Google Scholar 

  • Brederoo, P., Daems, W.Th.: Cell coat, worm-like structures, and labyrinths in guinea pig resident and exudate peritoneal macrophages, as demonstrated by an abbreviated fixation procedure for electron microscopy. Z. Zellforsch. 126, 135–156 (1972a)

    Google Scholar 

  • Brederoo, P., Daems, W.Th.: Unpublished observations (1972b)

  • Brederoo, P., Marani, E., Daems, W.Th.: Unpublished observations (1971)

  • Breton-Gorius, J., Guichard, J.: Etude au microscope électronique de la localisation de peroxydases dans les cellules de la moelle osseuse humaine. Nouv. Rev. franç. Hémat. 9, 678–687 (1969)

    Google Scholar 

  • Briggs, R.S., Perillie, P.E., Finch, S.C.: Lysozyme in bone marrow and peripheral blood cells. J. Histochem. Cytochem. 14, 167–170 (1966)

    Google Scholar 

  • Brökelmann, J., Fawcett, D.W.: The localization of endogenous peroxidase in the rat uterus and its induction by estradiol. Biol. Reproduct. 1, 59–71 (1969)

    Google Scholar 

  • Brücher, H., Dill, A., Gräber, M.: Untersuchungen über Blut- und Gewebsmakrophagen. Acta haemat. (Basel) 41, 76–93 (1969)

    Google Scholar 

  • Bültmann, B., Bigazzi, P.L., Heymer, B., Haferkamp, O.: Peritoneal macrophage disappearance reaction in the rat. Z. Immun.-Forsch. 142, 267–275 (1971)

    Google Scholar 

  • Burns, E.R., Soloff, B.L., Hanna, C., Buxton, D.F.: Nuclear pockets associated with the nucleolus in normal and neoplastic cells. Cancer Res. 31, 159–165 (1971)

    Google Scholar 

  • Carr, I.: The fine structure of cells of the mouse peritoneum. Z. Zellforsch. 80, 534–555 (1967)

    Google Scholar 

  • Carr, I.: Lysosome formation and surface changes in stimulated peritoneal cells. Z. Zellforsch. 89, 328–354 (1968)

    Google Scholar 

  • Carr, I.: The fine structure of the mammalian lymphoreticular system. Intern. Rev. Cytol. 27, 283–347 (1970)

    Google Scholar 

  • Cline, M.J.: Leucocyte function in inflammation: the ingestion, killing, and digestion of microorganisms. In: Aspects of Inflammation II (K.G. Jensen, S.A. Kilmann, eds.) Ser. Haematol. III/2, 3–16 (1970)

  • Cohn, Z.A.: The structure and function of monocytes and macrophages. Advanc. Immunol. 9, 163–214 (1968)

    Google Scholar 

  • Cotran, R.S., Litt, M.: The entry of granule-associated peroxidase into the phagocytic vacuoles of eosinophils. J. exp. Med. 129, 1291–1306 (1969)

    Google Scholar 

  • Cotran, R.S., Litt, M.: Ultrastructural localization of horseradish peroxidase and endogenous peroxidase activity in guinea pig peritoneal macrophages. J. Immunol. 105, 1536–1546 (1970)

    Google Scholar 

  • Crombie, P.R.: A new specialization of the granular endoplasmic reticulum in the uterine glands of the pig in the later stages of pregnancy. J. Cell Sci. 10, 693–704 (1972)

    Google Scholar 

  • Daems, W.Th.: Unpublished observations (1972)

  • Daems, W.Th., Brederoo, P.: The fine structure of mononuclear phagocytes as revealed by freeze-etching. In: Mononuclear phagocytes (R. van Furth, ed.), p. 29–42. Oxford-Edinburgh: Blackwell Sci. Publ. 1970 (a)

    Google Scholar 

  • Daems, W.Th., Brederoo, P.: Submicroscopic cytology of guinea pig peritoneal exudates. II. The peroxidase activity of the monocytes. In: Microscopie électronique 1970 (P. Favard, ed.). Rés. 7e Congr. Intern. Grenoble 1970. Vol. III, p. 543–544. Paris: Soc. Franç. Microsc. Electr. 1970 (b)

    Google Scholar 

  • Daems, W.Th., Brederoo, P.: The fine structure and peroxidase activity of resident and exudate peritoneal macrophages in the guinea pig. In: The reticuloendothelial system and immune phenomena (N. R. Di Luzio, K. Flemming, eds.). Proc. 6th Intern. Meeting Reticuloend. Soc. Freiburg 1970. Adv. exp. Med. Biol. 15, 19–31. New York: Plenum Press 1971

    Google Scholar 

  • Daems, W.Th., Cornelisse, C.J., Poelmann, R.E.: Interpretation of the results of metal salt methods for electron-microscopical enzyme cytochemistry. In: Electron microscopy 1972. Proc. 5th Europ. Congr. Electr. Microsc. Manchester 1972. p. 264–269. London-Bristol: The Institute of Physics 1972

    Google Scholar 

  • Daems, W.Th., Furth, R. van: Unpublished observations (1971)

  • Daems, W.Th., Poelmann, R.E., Brederoo, P.: Peroxidatic activity in resident peritoneal macrophages and exudate monocytes of the guinea pig after ingestion of latex particles. J. Histochem. Cytochem. 21, 93–95 (1973)

    Google Scholar 

  • Daems, W.Th., Wisse, E., Brederoo, P.: Electron microscopy of the vacuolar apparatus. In: Lysosomes in biology and pathology (J.T. Dingle, H.B. Fell, eds.), vol. I, p. 64–112. Amsterdam-London: North-Holland Publ. Comp. 1969

    Google Scholar 

  • Davis, A.T., Estensen, R., Quie, P.G.: Cytochalasin B. III. Inhibition of human polymorphonuclear leukocyte phagocytosis. Proc. Soc. exp. Biol. (N.Y.) 137, 161–164 (1971)

    Google Scholar 

  • Davis, R.H., McGowan, L.: Comparative peritoneal cellular content as related to species and sex. Anat. Rec. 162, 357–362 (1968)

    Google Scholar 

  • Douglas, S.D.: Disorders of neutrophil and monocyte function. Brit. J. Haemat. 21, 493–496 (1971)

    Google Scholar 

  • Dumont, A.: Ultrastructural study of the maturation of peritoneal macrophages in the hamster. J. Ultrastruct. Res. 29, 191–209 (1969)

    Google Scholar 

  • Dumont, A., Robert, M.D.: Electron microscopic study of phagocytosis of Histoplasma capsulum by hamster peritoneal macrophages. Lab. Invest. 23, 278–286 (1970)

    Google Scholar 

  • Dumont, A., Sheldon, H.: Changes in the fine structure of macrophages in experimentally produced tuberculous granulomas in hamsters. Lab. Invest. 14, 2034–2055 (1965)

    Google Scholar 

  • Dupuy-Coin, A.M., Bouteille, M.: Developmental pathway of granular and beaded nuclear bodies from nucleoli. J. Ultrastruct. Res. 40, 55–67 (1972)

    Google Scholar 

  • Ebert, R.H., Florey, H.W.: The extravascular development of the monocyte observed in vivo. Brit. J. exp. Path. 20, 342–356 (1939)

    Google Scholar 

  • Emeis, J.J., Wisse, E.: Electron microscopic cytochemistry of the cell coat of Kupffer cells in rat liver. In: The reticuloendothelial system and immune phenomena (N.R. Di Luzio, K. Flemming, eds.). Proc. 6th Intern. Meeting Reticuloend. Soc. Freiburg 1970. Adv. exp. Med. Biol. 15, 1–12. New York: Plenum Press 1971

    Google Scholar 

  • Enomoto, T., Kitani, T.: Electron microscopic studies on peroxidase and acid phosphatase reaction in human leukocytes. (In normal and leukemic cells and on the phagocytosis). Acta haemat. jap. 29, 554–570 (1966)

    Google Scholar 

  • Evans, W.H., Rechcigl, M.: Factors influencing myeloperoxidase and catalase activities in polymorphonuclear leukocytes. Biochim. biophys. Acta (Amst.) 148, 243–250 (1967)

    Google Scholar 

  • Everett, N.B., Tyler, R.W.: Studies of lymphocytes: Relationship to mononuclear cells of inflammatory exudates. Biochem. Pharmacol. Suppl. March 1968. p. 185–196

  • Fahimi, H.D.: Cytochemical localization of peroxidatic activity of catalase in rat hepatic microbodies (peroxisomes). J. Cell Biol. 43, 275–288 (1969)

    Google Scholar 

  • Fahimi, H.D.: The fine structural localization of endogenous and exogenous peroxidase activity in Kupffer cells of rat liver. J. Cell Biol. 47, 247–262 (1970)

    Google Scholar 

  • Fedorko, M.E., Hirsch, J.G.: Structure of monocytes and macrophages. Sem. Haematol. 7, 109–124 (1970)

    Google Scholar 

  • Fliedner, T.M., Cronkite, E.P., Robertson, J.S.: Granulocytopoiesis. I. Senescence and random loss of neutrophilic granulocytes in human beings. Blood 24, 402–414 (1964)

    Google Scholar 

  • Franke, W.W., Kartenbeck, J., Zentgraf, H., Scheer, U., Falk, H.: Membrane-to-membrane cross-bridges (a means to orientation and interaction of membrane faces). J. Cell Biol. 51, 881–888 (1971)

    Google Scholar 

  • Frederik, S.E., Newcomb, E.H.: Cytochemical localization of catalase in leaf microbodies (peroxisomes). J. Cell Biol. 43, 343–353 (1969)

    Google Scholar 

  • Furth, R. van: Origin and kinetics of monocytes and macrophages. Sem. Haematol. 7, 125–141 (1970a)

    Google Scholar 

  • Furth, R. van: Remark in the discussion. In: Mononuclear phagocytes (R. van Furth, ed.), p. 176. Oxford-Edinburgh: Blackwell Sci. Publ. 1970b

    Google Scholar 

  • Furth, R. van, Cohn, Z.A.: The origin and kinetics of mononuclear phagocytes. J. exp. Med. 128, 415–435 (1968)

    Google Scholar 

  • Furth, R. van, Cohn, Z.A., Hirsch, J.G., Humphrey, J.H., Spector, W.G., Langevoort, H.L.: The mononuclear phagocyte system: a new classification of macrophages, monocytes and their precursor cells. Bull. Wld Hlth Org. 46, 845–852 (1972)

    Google Scholar 

  • Furth, R. van, Hirsch, J.G., Fedorko, M.E.: Morphology and peroxidase cytochemistry of mouse promonocytes, monocytes and macrophages. J. exp. Med. 132, 794–812 (1970)

    Google Scholar 

  • Gee, J.B.L., Vassallo, C.L., Bell, P., Kaskin, J., Basford, R.E., Field, J.B.: Catalase-dependent peroxidative metabolism in the alveolar macrophage during phagocytosis. J. clin. Invest. 49, 1280–1287 (1970)

    Google Scholar 

  • Gillette, R.W., Lance, E.M.: Kinetic studies of macrophages. I. Distributional characteristics of radiolabeled peritoneal cells. J. reticuloendoth. Soc. 10, 223–237 (1971)

    Google Scholar 

  • Glick, A.D., Getnick, R.A., Cole, R.M.: Electron microscopy of group A streptococci after phagocytosis by human monocytes. Infect. Immun. 4, 772–779 (1971)

    Google Scholar 

  • Goldfischer, S., Villaverde, H., Forschrim, R.: The demonstration of acid hydrolase, thermostable reduced diphosphopyridine nucleotide tetrazolium reductase and peroxidase activities in human lipofuscin pigment granules. J. Histochem. Cytochem. 14, 641–652 (1966)

    Google Scholar 

  • Hanaoka, H., Friedman, B.: Paired cisternae in human tumor cells. J. Ultrastruct. Res. 32, 323–333 (1970)

    Google Scholar 

  • Hard, G.C.: Electron microscopic study of the differentiation of mouse peritoneal macrophages stimulated by Corynebacterium ovis infection. Lab. Invest. 21, 309–315 (1969)

    Google Scholar 

  • Helden, H.P.T. van: Ontstekingscellen en de afbraak van antigeen-antilichaam precipitaat. Thesis Leiden, 1971

  • Herzog, V., Fahimi, H.D.: Glutaraldehyde fixation: a prerequisite for demonstration of the peroxidatic activity of catalase in isolated rat liver peroxisomes and crystalline beef liver catalase. J. Cell Biol. 55, 113a (1972)

  • Herzog, V., Miller, F.: Die Lokalisation endogener Peroxidase in der Glandula parotis der Ratte. Z. Zellforsch. 107, 403–420 (1970)

    Google Scholar 

  • Herzog, V., Miller, F.: The localization of endogenous peroxidase in the lacrimal gland of the rat during postnatal development. J. Cell Biol. 53, 662–680 (1972a)

    Google Scholar 

  • Herzog, V., Miller, F.: Endogenous peroxidase in the lacrimal gland of the rat and its differentiation against injected catalase and horseradish-peroxidase. Histochemie 30, 235–246 (1972b)

    Google Scholar 

  • Hibbs, J.B., Lambert, L.H., Remington, J.S.: Possible role of macrophage mediated non-specific cytotoxicity in tumour resistance. Nature (Lond.) New Biol. 235, 48–50 (1972)

    Google Scholar 

  • Hirsch, J.G., Fedorko, M.E.: Ultrastructure of human leukocytes after simultaneous fixation with glutaraldehyde and osmium tetroxide and “postfixation” in uranyl acetate. J. Cell Biol. 38, 615–627 (1968)

    Google Scholar 

  • Horn, R.G., Koenig, M.G., Goodman, S., Collins, R.D.: Phagocytosis of Staphylococcus aureus by hepatic reticuloendothelial cells. An ultrastructural study. Lab. Invest. 21, 406–414 (1969)

    Google Scholar 

  • Hruban, Z., Rechcigl, M.: Microbodies and related particles. Morphology, biochemistry, and physiology. Int. Rev. Cytol. Suppl. I, 1969

  • Huhn, D., Schmalzl, F., Demmler, K.: Monozytenleukämie. Dtsch. med. Wschr. 96, 1594–1605 (1971)

    Google Scholar 

  • Jones, T.C., Hirsch, J.G.: The interaction in vitro of Mycoplasma pulmonis with mouse peritoneal macrophages and -cells. J. exp. Med. 133, 231–259 (1971)

    Google Scholar 

  • Joos, F., Roos, B., Bürki, H., Bürki, K., Laissue, J.: Umsatz, Proliferation und Phagozytosetätigkeit der freien Zellen im Peritonäalraum der Maus nach Injektion von Polystyren-Partikeln. Z. Zellforsch. 95, 68–85 (1969)

    Google Scholar 

  • Karnovsky, M.L., Simmons, S., Glass, E.A., Shafer, A.W., D'Arcy Hart, P.: Metabolism of macrophages. In: Mononuclear phagocytes (R. van Furth, ed.), p. 103–120. Oxford-Edinburgh: Blackwell Sci. Publ. 1970

    Google Scholar 

  • Klebanoff, S.J.: Myeloperoxidase-mediated antimicrobial systems and their role in leukocyte function. In: Biochemistry of the phagocytic process (J. Schultz, ed.), p. 89–110. Amsterdam: North-Holland Publ. Co. 1970

    Google Scholar 

  • Klebanoff, S.J.: Intraleukocytic microbicidal defects. Ann. Rev. Med. 22, 39–67 (1971)

    Google Scholar 

  • Klebanoff, S.J., Hamon, C.B.: Role of myeloperoxidase-mediated antimicrobial systems in intact leukocytes. J. reticuloendoth. Soc. 12, 170–196 (1972)

    Google Scholar 

  • Kolin, A., Landi, S.: Phagocytosis of polymorphonuclear leukocytes by spleen macrophages in tuberculin fever. Amer. Rev. resp. Dis. 101, 728–740 (1970)

    Google Scholar 

  • Kondo, Y.: Macrophages containing Langerhans cell granules in normal lymph nodes of the rabbit. Z. Zellforsch. 4, 506–511 (1969)

    Google Scholar 

  • Leake, E.S., Heise, E.R.: Comparative cytology of alveolar and peritoneal macrophages from germ free rats. In: The reticuloendothelial system and atherosclerosis (N.R. Di Luzio, R. Paoletti, eds.), p. 133–146. New York: Plenum Press 1967

    Google Scholar 

  • Leder, L.D.: Der Blutmonozyt. Berlin-Heidelberg-New York: Springer 1967

    Google Scholar 

  • Lennert, K., Leder, L.D.: The origin of the macrophages in the “skin windows”. In: The reticuloendothelial system. Morphology, immunology and regulation. Proc. IVth Intern. Symp. RES, Otsu and Kyoto 1964. p. 25–32. Jap. Soc. Promot. Sci. Tokyo 1964

  • Lennert, K., Niedorf, H.R., Blümcke, S.: Lymphadenitis with massive hemophagocytic sinus histiocytosis. Virchows Arch. Abt. B 10, 14–29 (1972)

    Google Scholar 

  • Lentz, P.E., Di Luzio, N.R.: Biochemical characterization of Kupffer and parenchymal cells isolated from rat liver. Exp. Cell Res. 67, 17–26 (1971)

    Google Scholar 

  • Luft, J.H.: Ruthenium red and violet. II. Fine structural localization in animal tissues. Anat. Rec. 171, 369–416 (1971)

    Google Scholar 

  • Mackaness, G.B.: The phagocytosis and inactivation of staphylococci by macrophages of normal rabbits. J. exp. Med. 112, 35–53 (1960)

    Google Scholar 

  • McLaughlin, J.F., Ruddle, N.H., Waksman, B.H.: Relationship between activation of peritoneal cells and their cytopathogenicity. J. reticuloendoth. Soc. 12, 293–304 (1972)

    Google Scholar 

  • Michell, R.H., Karnovsky, M.J., Karnovsky, M.L.: The distributions of some granule-associated enzymes in guinea pig polymorphonuclear leucocytes. Biochem. J. 116, 207–216 (1970)

    Google Scholar 

  • Miller, T.E.: Metabolic events involved in the bactericidal activity of normal mouse macrophages. Infect. Immun. 3, 390–397 (1971)

    Google Scholar 

  • Morikawa, S., Harada, T.: Immunohistochemical localization of catalase in mammalian tissues. J. Histochem. Cytochem. 17, 30–35 (1969)

    Google Scholar 

  • Myrvik, Q.N., Leake, E.S., Fariss, B.: Lysozyme content of alveolar and peritoneal macrophages from the rabbit. J. Immunol. 86, 133–136 (1961)

    Google Scholar 

  • Nichols, B.A., Bainton, D.F., Farquhar, M.G.: Differentiation of monocytes. Origin, nature and fate of their azurophil granules. J. Cell Biol. 50, 498–515 (1971)

    Google Scholar 

  • North, R.J.: The relative importance of blood monocytes and fixed macrophages to the expression of cell-mediated immunity to infection. J. exp. Med. 132, 521–534 (1970)

    Google Scholar 

  • North, R.J., Mackaness, G.B.: Electronmicroscopical observations on the peritoneal macrophages of normal mice and mice immunised with Listeria monocytogenes. I. Structure of normal macrophages and the early cytoplasmic response to the presence of ingested bacteria. Brit. J. exp. Path. 44, 601–706 (1963)

    Google Scholar 

  • Novikoff, A.B., Beard, M.E., Albala, A., Sheid, B., Quintana, N., Biempica, L.: Localization of endogenous peroxidases in animal tissues. J. Microscopie 12, 381–404 (1971)

    Google Scholar 

  • Novikoff, A.B., Biempica, L., Quintana, N., Albala, A., Dominitz, R.: Peroxidatic activity in cell organelles. J. Cell Biol. 39, 100a (1968)

  • Novikoff, A.B., Goldfischer, S.: Visualization of peroxisomes (microbodies) and mitochondria with diaminobenzidine. J. Histochem. Cytochem. 17, 675–680 (1969)

    Google Scholar 

  • Novikoff, P.M., Novikoff, A.B.: Peroxisomes in absorptive cells of mammalian small intestine. J. Cell Biol. 53, 532–560 (1972)

    Google Scholar 

  • Padawer, J., Gordon, A.S.: Cellular elements in the peritoneal fluid of some mammals. Anat. Rec. 124, 209–222 (1956)

    Google Scholar 

  • Parwaresch, M.R., Müller-Hermelink, H.K., Desaga, J.F., Zakari, V., Lennert, K.: Die Herkunft der Gewebsmastzellen bei der Ratte, zugleich ein Beitrag zur quantitativen Cytologie der sterilen Peritonitis. Virchows Arch. Abt. B 8, 20–35 (1971)

    Google Scholar 

  • Paul, B.B., Strauss, R.R., Jacobs, A.A., Sbarra, A.J.: Function of H2O2, myeloperoxidase, and hexose monophosphate shunt enzymes in phagocytizing cells from different species. Infect. Immun. 1, 338–344 (1970)

    Google Scholar 

  • Pearsall, N.N., Weiser, R.S.: The macrophage. Philadelphia: Lea & Feabiger 1970

    Google Scholar 

  • Petrik, P., Collet, A.J.: Electron microscopic study of the in vivo erythrophagocytosis by alveolar macrophages of the cat. II. Late period: digestion. Z. Zellforsch. 116, 477–486 (1971)

    Google Scholar 

  • Petris, S. de, Karlsbad, G., Pernis, B.: Filamentous structures in the cytoplasm of normal mononuclear phagocytes. J. Ultrastruct. Res. 7, 39–55 (1962)

    Google Scholar 

  • Pinkett, M., Olivia, M.S., Cowdrey, C.R., Nowell, P.C.: Mixed hematopoietic and pulmonary origin of alveolar macrophages as demonstrated by chromosome markers. Amer. J. Path. 48, 859–865 (1966)

    Google Scholar 

  • Poelmann, R.E., Daems, W.Th.: Problems associated with the demonstration by lead methods of ATP-ase activity in resident peritoneal macrophages and exudate monocytes of the guinea pig. J. Histochem. Cytochem. 488–498 (1973)

  • Pratt, S.A., Smith, M.H., Ladman, A.J., Finley, T.N.: The ultrastructure of alveolar macrophages from human cigarette smokers and nonsmokers. Lab. Invest. 24, 331–338 (1971)

    Google Scholar 

  • Robbins, D., Fahimi, H.D., Cotran, R.S.: Fine structural cytochemical localization of peroxidase activity in rat peritoneal cells: mononuclear cells, eosinophils and mast cells. J. Histochem. Cytochem. 19, 571–575 (1971)

    Google Scholar 

  • Robineaux, R., Anteunis, A., Bona, C.: Ultrastructure des macrophages de cobaye. Ann. Inst. Pasteur 120, 329–336 (1971)

    Google Scholar 

  • Roels, F.: Localisation d'activités peroxydasiques dans l'oeuf d'Artemia salina, à l'aide de 3,3′-diaminobenzidine, de benzidine et de pyrogallol. Arch. Biol. (Liège) 81, 229–274 (1970)

    Google Scholar 

  • Roels, F., Wisse, E.: Distinction cytochimique entre catalase et peroxidases. C. R. Acad. Sci. (Paris) Serie D 276, 391–393 (1973)

    Google Scholar 

  • Roos, B.: Makrophagen: Herkunft, Entwicklung und Funktion. In: Handbuch der allgemeinen Pathologie, Bd. VII/3 (A. Studer, H. Cottier, Hrsg.) S. 1–128. Berlin-Heidelberg-New York: Springer 1970

    Google Scholar 

  • Roser, B.: The origin, kinetics and fate of macrophage populations. J. reticuloendoth. Soc. 8, 139–161 (1970)

    Google Scholar 

  • Rossi, F., Zatti, M., Patriarca, P.: H2O2 production during NADPH oxidation by the granule fraction of phagocytosing polymorphonuclear leucocytes. Biochim. biophys. Acta (Amst.) 184, 201–203 (1969)

    Google Scholar 

  • Rowley, D.: Phagocytosis. In: Advances in immunology W.H. Taliaferro, J.H. Humphrey, (eds.), vol. 2, p. 241–264. New York-London: Acad. Press 1962

    Google Scholar 

  • Sbarra, A.J., Paul, B.B., Strauss, R.R., Jacobs, A.A., Mitchell, G.W.: The biochemical and antimicrobial activities of the phagocyte. In: The reticulo endothelial system and immune phenomena (N. R. Di Luzio, K. Flemming, eds.). Proc. 6th Intern. Meeting Reticuloend. Soc. Freiburg 1970. Adv. exp. Med. Biol. 15, 209–219. New York: Plenum Press 1971

    Google Scholar 

  • Shah, D.O.: Interaction of uranyl ions with phospholipid and cholesterol monolayers. J. Colloid Interface Sci. 29, 210–215 (1969)

    Google Scholar 

  • Shirahama, T., Cohen, A.S.: Lysosomal breakdown of amyloid fibrils by macrophages. Amer. J. Path. 63, 463–478 (1971)

    Google Scholar 

  • Silva, M.T., Carvalho Guerra, F., Magalhaes, M.M.: The fixative action of uranyl acetate in electron microscopy. Experientia (Basel) 24, 1074 (1968)

    Google Scholar 

  • Slonecker, C.E.: The cellular composition of an acute inflammatory exudate in rats. J. reticuloendoth. Soc. 10, 269–282 (1971)

    Google Scholar 

  • Smith, C.W., Goldman, A.S.: Effects of concanavalin A and pokeweed mitogen in vivo on mouse peritoneal macrophages. Exp. Cell Res. 73, 394–398 (1972)

    Google Scholar 

  • Spooner, B.S., Yamada, K.M., Wessells, N.K.: Microfilaments and cell locomotion. J. Cell Biol. 49, 595–613 (1971)

    Google Scholar 

  • Sutton, J.S.: Ultrastructural aspects of in vitro development of monocytes into macrophages, epitheloid cells, and multinucleated giant cells. Nat. Cancer Inst. Monogr. 26, 71–141 (1967)

    Google Scholar 

  • Taichman, N.S., Uriuhara, T., Movat, H.Z.: Ultrastructural alterations in the local Shwartzman reaction. Lab. Invest. 14, 2160–2176 (1966)

    Google Scholar 

  • Terzakis, J.A.: Uranyl acetate, a stain and a fixative. J. Ultrastruct. Res. 22, 168–184 (1968)

    Google Scholar 

  • Unanue, E.R., Cerottini, J.C.: The function of macrophages in the immune response. Sem. Haematol. 7, 225–248 (1970)

    Google Scholar 

  • Volkman, A.: The origin and fate of the monocyte. In: Aspects of inflammation. II. (K.G. Jensen, S.A. Killmann, eds.). Ser. Haematol. III/2, 62–92 (1970)

  • Volkman, A.: A current perspective of monocytopoiesis. Curr. Top. Pathol. 54, 76–94 (1971)

    Google Scholar 

  • Vye, M.V., Fischman, D.A.: The morphological alteration of particulate glycogen by en bloc staining with uranyl acetate. J. Ultrastruct. Res. 33, 278–291 (1970)

    Google Scholar 

  • Watanabe, K., Enomoto, Y.: Ultrastructural localization of peroxidase in mononuclear phagocytes from the peritoneal cavity, blood, bone marrow and omentum: origin of the peritoneal macrophages. In: Histochemistry, cytochemistry 1972. (T. Takeuchi, K. Ogawa, S. Fujita, eds.), p. 433–434. Proc. Fourth Intern. Congr. Histochem. Cytochem., Kyoto 1972

  • Wessels, N.K., Spooner, B.S., Ash, J.F., Bradley, M.O., Luduena, M.A., Taylor, E.L., Wrenn, J.T., Yamada, K.M.: Microfilaments in cellular and developmental processes. Science 171, 135–143 (1971)

    Google Scholar 

  • Whitelaw, D.M.: The intravascular lifespan of monocytes. Blood 28, 455–464 (1966)

    Google Scholar 

  • Whitelaw, D.M., Batho, H.F.: The distribution of monocytes in the rat. Cell Tiss. Kinet. 5, 215–225 (1972)

    Google Scholar 

  • Widmann, J.J., Cotran, R.S., Fahimi, H.D.: Mononuclear phagocytes (Kupffer cells) and endothelial cells. Identification of two functional cell types in rat liver sinusoids by endogenous peroxidase activity. J. Cell Biol. 52, 159–170 (1972)

    Google Scholar 

  • Widmann, J.J., Fahimi, H.D.: Mitotic activities of Kupffer cells and endothelial cells in regenerating rat liver. J. Cell Biol. 55, 279a (1972)

  • Willighagen, R.G.J., Daems, W.Th.: Unpublished observations (1972)

  • Wisse, E.: An electron microscopic study of the fenestrated endothelial lining of rat liver sinusoids. J. Ultrastruct. Res. 31, 125–150 (1970)

    Google Scholar 

  • Wisse, E.: An ultrastructural characterization of the endothelial cell in the rat liver sinusoid under normal and various experimental conditions, as a contribution to the distinction between endothelial and Kupffer cells. J. Ultrastruct. Res. 38, 528–562 (1972)

    Google Scholar 

  • Wisse, E.: Unpublished observations (1973)

  • Wisse, E., Daems, W.Th.: Fine structural study on the sinusoidal lining cells of rat liver. In: Mononuclear phagocytes (R. van Furth, ed.), p. 200–210. Oxford-Edinburgh: Black-well Sci. Publ. 1970

    Google Scholar 

  • Wisse, E., Giphardt, M., Meulen, J. van der, Roels, F., Emeis, J.J., Daems, W.Th.: Peroxidatic activity in bacteria-phagocytizing Kupffer cells, demonstrated by a new method: perfusion incubation. In: Electron microscopy 1972. Proc. 5th Europ. Congr. Electr. Microsc. Manchester 1972. p. 272–273. London-Bristol: The Institute of Physics 1972

    Google Scholar 

  • Wohlfarth-Bottermann, K.E., Komnick, H.: Die Gefahren der Glutaraldehydfixation. J. Microscopie 5, 441–452 (1966)

    Google Scholar 

  • Wooding, F.B.P.: The mechanism of secretion of the milk fat globule. J. Cell Sci. 9, 805–821 (1971)

    Google Scholar 

  • Zucker-Franklin, D., Hirsch, J.G.: Electron microscope studies on the degranulation of rabbit peritoneal leukocytes during phagocytosis. J. exp. Med. 120, 569–575 (1964)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory for Electron Microscopy, University of Leiden, The Netherlands

    W.Th. Daems & P. Brederoo

Authors
  1. W.Th. Daems
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Brederoo
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

With the assistance of E.J. van Lohuizen.

Acknowledgements. The authors are greatly indebted to Mrs. E.G.J. Meeuwissen-van der Vlugt, Mr. L. Verschragen, and Mr. J. Beentjes for their skillful technical assistance and to Drs. E. Wisse and J. J. Emeis for their discussion of the manuscript.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Daems, W., Brederoo, P. Electron microscopical studies on the structure, phagocytic properties, and peroxidatic activity of resident and exudate peritoneal macrophages in the guinea pig. Z.Zellforsch 144, 247–297 (1973). https://doi.org/10.1007/BF00307305

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00307305

Key words

Search

Navigation