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Effects of ovarian hormones on cell membranes in the rat uterus

I. Freeze fracture studies of the apical membrane of the luminal epithelium

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Abstract

Freeze fracture techniques have been used to study the apical membrane of cells of the luminal epithelium of the rat uterus under various hormonal regimes. In the ovariectomized but otherwise untreated rat, intramembranous particles (IMPs) occur at a density of 1395±122 per μm2; they appeared spherical and uniformly distributed. After 3 days treatment with estrogen, no change in appearance or density was found, but 3 days of progesterone treatment produced a significant increase in IMP density to 1622±104. Treatment with progesterone, with an additional dose of estrogen on day 3, is known to produce an epithelium receptive to the implanting blastocyst. In these conditions, the IMP density rose to 3818±337; rod-shaped particles and aggregations of IMPs were seen, and some particle arrays resembling gap junctions, in addition to the isolated spherical particles.

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Authors and Affiliations

  1. School of Medicine, Flinders University of South Australia, 5042, Bedford Park, S.A., Australia

    C. R. Murphy & A. W. Rogers

  2. Electron Microscope Unit, Tissue Pathology Division, Institute of Medical and Veterinary Science, Adelaide, South Australia

    J. G. Swift & T. M. Mukherjee

Authors
  1. C. R. Murphy
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  2. J. G. Swift
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  3. T. M. Mukherjee
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  4. A. W. Rogers
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Murphy, C.R., Swift, J.G., Mukherjee, T.M. et al. Effects of ovarian hormones on cell membranes in the rat uterus. Cell Biochem Biophys 1, 181–193 (1979). https://doi.org/10.1007/BF02781350

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  • DOI: https://doi.org/10.1007/BF02781350

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