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Production of cellulase on mixtures of xylose and cellulose

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Abstract

Cellulase production by the RUT-C30 mutant of the fungusTrichoderma reesei was studied on mixtures of xylose and cellulose. In mixed substrates, the lag phase of the growth cycle was shorter and reached the maximum of total productivity in a shorter time compared to growth on the single substrate, cellulose. A diauxic pattern of utilization of the two carbon sources was observed as well: Xylose was utilized first to support growth, followed by cellulose to induce the cellulase enzyme production and provide an additional carbon source for cellular metabolism. Of the various mixtures of xylose and cellulose used in batch enzyme production, a ratio of 30∶30 g/L of xylose to cellulose was optimal. This mixture produced the highest maximal enzyme productivity of 122 IFPU/L h, and its total productivity reached a maximum value of 55 IFPU/L h in less time than others. However, similar total productivities and higher enzyme titers were observed for growth on cellulose alone.

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  1. Biotechnology Branch, Solar Energy Research Institute, 1617 Cole Blvd., 80401, Golden, CO

    Ali Mohagheghi, Karel Grohmann & Charles E. Wyman

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  1. Ali Mohagheghi
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  2. Karel Grohmann
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  3. Charles E. Wyman
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Mohagheghi, A., Grohmann, K. & Wyman, C.E. Production of cellulase on mixtures of xylose and cellulose. Appl Biochem Biotechnol 17, 263–277 (1988). https://doi.org/10.1007/BF02779162

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