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A protocol for rapid DNA extraction fromArabidopsis thaliana for PCR analysis

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Abstract

We present a method for instant DNA extraction fromArabidopsis thaliana based on a simple DNA extraction method (Edwards et al., 1991). A piece of rosette leaf (typically 3–5 mg) was ground in a centrifuge tube in extraction solution. Extracted DNA was suitable for PCR analysis, without centrifugation. The feasibility of this method was confirmed by testing 24 primer sets. This method requires less than 1 mg of plant tissue and is useful for genetic mapping, transgene detection, and other experiments.

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Abbreviations

EDTA:

ethylenediaminetetraacetic acid

WT:

wild type

References

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Author notes

  1. Naoko Ohkama-Ohtsu

    Present address: Department of Genetics, Development and Cell Biology, Iowa State University, 50011, Ames, IA, USA

Authors and Affiliations

  1. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan

    Ichiro Kasajima, Naoko Ohkama-Ohtsu, Hiroaki Hayashi & Tadakatsu Yoneyama

  2. Biotechnology Research Center, The University of Tokyo, Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan

    Yoko Ide & Toru Fujiwara

  3. PRESTO, JST, Japan

    Toru Fujiwara

Authors
  1. Ichiro Kasajima
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  2. Yoko Ide
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  3. Naoko Ohkama-Ohtsu
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  4. Hiroaki Hayashi
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  5. Tadakatsu Yoneyama
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  6. Toru Fujiwara
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Kasajima, I., Ide, Y., Ohkama-Ohtsu, N. et al. A protocol for rapid DNA extraction fromArabidopsis thaliana for PCR analysis. Plant Mol Biol Rep 22, 49–52 (2004). https://doi.org/10.1007/BF02773348

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  • DOI: https://doi.org/10.1007/BF02773348

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