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Simple procedure for estimating the efficiency of PCR

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Abstract

The extension efficiency of PCR-driven DNA amplification was examined by step-controlled limiting dilution method using as internal controls 8E5 lymphocytes carrying a single copy of HIV genome. The results reveal that under standard conditions recommended by the PCR kit manufacturer the exponential growth of DNA template is approximately one third of the theoretical doubling rate.

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Authors and Affiliations

  1. Metatron Inc., 31 Stuyvesant St., 10003, New York, NY

    Aldar S. Bourinbaiar

  2. Zanexis Inc., Malvern, PA

    Kenneth Ajuang-Simbiri

Authors
  1. Aldar S. Bourinbaiar
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  2. Kenneth Ajuang-Simbiri
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Bourinbaiar, A.S., Ajuang-Simbiri, K. Simple procedure for estimating the efficiency of PCR. Mol Biotechnol 6, 87–89 (1996). https://doi.org/10.1007/BF02762328

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  • DOI: https://doi.org/10.1007/BF02762328

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