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Akkurate Bestimmung der Plasmidkopienzahl pro Zelle

  • Biotechnologie
  • Droplet Digital PCR
  • Published:
BIOspektrum Aims and scope

Abstract

Plasmids are circular DNA molecules that are used as gene vectors in countless applications. They are easy to manipulate and transfer, but can also vary considerably in copy number between individual cells. In this article, a model expression system with a large amount of heterogeneity is presented. We used flow cytometry and digital PCR to count the number of plasmids in sorted sub-populations. Finally, heterogeneity could be attributed to the expression plasmid being either present or absent in bacterial sub-populations.

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Authors and Affiliations

  1. Department Umweltmikrobiologie AG Flow Cytometry, Helmholtz-Zentrum für Umweltforschung GmbH – UFZ, Permoserstraße 15, D-04318, Leipzig, Deutschland

    Michael Jahn, Carsten Vorpahl, Dominique Türkowsky & Susann Müller

Authors
  1. Michael Jahn
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  2. Carsten Vorpahl
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  3. Dominique Türkowsky
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  4. Susann Müller
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Corresponding author

Correspondence to Susann Müller.

Additional information

Michael Jahn, Carsten Vorpahl, Dominique Türkowsky, Susann Müller (v. l. n. r.)

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Jahn, M., Vorpahl, C., Türkowsky, D. et al. Akkurate Bestimmung der Plasmidkopienzahl pro Zelle. Biospektrum 22, 211–213 (2016). https://doi.org/10.1007/s12268-016-0674-3

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  • DOI: https://doi.org/10.1007/s12268-016-0674-3

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