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HPLC determination of phytoplankton pigments using N,N-dimethylformamide

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Abstract

The suitability of N,N-dimethylformamide (DMF) as an extractant for the standard reverse-phase HPLC method was examined using algal cultures. Good pigment separations and recovery were achieved with 20% (volume %) addition of an ion-pairing solution in an injection. While slight amounts of degradation products of chlorophylla, i.e., chlorophyllidea, allomeric and epimeric forms, were produced, adequate attention to filtration and extraction prevents the formation of degradation products, confining them to an acceptable level. Because of its strong extractability, which expedites the extraction process, DMF is an efficient solvent for HPLC analysis of phytoplankton pigments.

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Author notes

  1. Yasushi Yabushita

    Present address: Akagi Suisan Co., 3211, Handa, Shibukawa, 377, Gunma, Japan

Authors and Affiliations

  1. Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi, Bunkyo, 113-8657, Tokyo, Japan

    Ken Furuya & Masato Hayashi

  2. Faculty of Bioresources, Mié University, Kamihama, Tsu, 514-0008, Mié, Japan

    Yasushi Yabushita

Authors
  1. Ken Furuya
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  2. Masato Hayashi
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  3. Yasushi Yabushita
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Furuya, K., Hayashi, M. & Yabushita, Y. HPLC determination of phytoplankton pigments using N,N-dimethylformamide. J Oceanogr 54, 199–203 (1998). https://doi.org/10.1007/BF02751695

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  • DOI: https://doi.org/10.1007/BF02751695

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