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Localization of activin A/EDF in the normal and diabetic rat pancreas: Immunohistochemical andIn Situ hybridization studies

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Abstract

We examined the immunohistochemical localization of activin A/erythroid differentiation factor (EDF) in the pancreases of normal and diabetic rats with insulin-dependent diabetes mellitus or noninsulin-dependent diabetes mellitus to elucidate how activin A/EDF modulates insulin secretion. In both the normal and the diabetic pancreas, all of the cells staining with antirecombinant human (rh) activin A/EDF antiserum were insulin-producing B-cells, and they gradually decreased in number with the development of diabetic changes in both types of diabetic rats. No rh activin A/EDF immunoreactivity was detected in A-, D-, or pancreatic polypeptide (PP) cells in the islets, or in the exocrine cells. Thein situ hybridization (ISH) method showed that activin A/EDF mRNA is localized in activin A/EDF-positive cells. These data indicated that activin A/EDF localizes in insulin-producing B-cells of normal and diabetic pancreases, and may act as an autocrine modulator of insulin secretion.

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Authors and Affiliations

  1. First Department of Pathology, School of Medicine, The University of Tokushima, Japan

    Toshiaki Sano MD, PhD

  2. First Department of Internal Medicine, School of Medicine, The University of Tokushima, Kuramoto-cho 3, 770, Tokushima, Japan

    Makoto Ide MD, Yasumi Shintani MD, PhD, Masaaki Kosaka MD, PhD & Shiro Saito MD, PhD

Authors
  1. Makoto Ide MD
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  2. Yasumi Shintani MD, PhD
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  3. Masaaki Kosaka MD, PhD
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  4. Toshiaki Sano MD, PhD
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  5. Shiro Saito MD, PhD
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Ide, M., Shintani, Y., Kosaka, M. et al. Localization of activin A/EDF in the normal and diabetic rat pancreas: Immunohistochemical andIn Situ hybridization studies. Endocr Pathol 7, 229–236 (1996). https://doi.org/10.1007/BF02739926

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  • DOI: https://doi.org/10.1007/BF02739926

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