Skip to main content
SpringerLink
Log in

Inheritance and segregation of exogenous genes in transgenic cotton

  • Published:
Journal of Genetics Aims and scope Submit manuscript

Abstract

Three transgenic cotton varieties (lines) were chosen for the study of inheritance and segregation of foreign Bt (Bacillus thuringiensis toxin) andtfdA genes in cotton. The transformed cotton varieties CCRI 30 and NewCott 33B expressing the BtcryIA gene, and cotton line TFD expressing thetfdA gene were crossed with CCRI 19, CCRI 12 and Lumian 6. The results confirm inheritance and segregation of (i) the exogenous Bt gene in transgenic CCRI 30 and NewCott 33B, governing resistance to bollworm, and (ii) the exogenoustfdA gene in transgenic TFD, governing resistance to the herbicide 2,4-D. Both resistance characters were governed by a single dominant nuclear gene, and were not affected by cytoplasm. Our data support the conclusion that foreign traits encoded by single genes are inherited and expressed in Mendelian fashion in cotton. Our results also indicate that a practical backcross breeding program could be used to develop cotton cultivars combining one or more resistance traits from foreign and native gene sources.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bayley C., Trolinder N., Ray C., Morgan M. M., Quisenberry J. E. and Ow D. W. 1992 Engineering 2,4-D resistance into cotton.Theor. Appl. Genet. 83, 645–649.

    Article  CAS  Google Scholar 

  • Benedict J. H., Altman D. W., Umbeck P. F. and Ring D. R. 1992 Behavior, growth, survival, and plant injury forHeliothis virescens (F.) (Lepidoptera: Noctuidae) on transgenic Bt cottons.J. Econ. Entomol. 85, 589–593.

    Google Scholar 

  • Benedict J. H., Sachs E. S., Altman D. W., Deaton W. R., Kohel R. J., Ring D. R. and Berberich S. A. 1996 Field performance of cottons expressing transgenic Cry IA insecticidal proteins for resistance toHeliothis virescens andHelicoverpa zea (Lepidoptera: Noctuidae) on transgenic Bt cottons.J. Econ. Entomol. 89, 230–238.

    Google Scholar 

  • Chen Z. X., Llewellyn D. J., Fan Y. L., Li S. J., Guo S. D., Jiao G. L. and Zhao J. X. 1994 2,4-D resistant transgenic cotton plants produced byAgrobacterium-mediated gene transfer.Sci. Agric. Sinica 27, 31–37.

    CAS  Google Scholar 

  • Cousins Y. L., Lyon B. R. and Llewellyn D. J. 1991 Transformation of an Australian cotton cultivar: prospects for cotton improvement through genetic engineering.Aus. J. Plant Physiol. 18, 481–494.

    Article  CAS  Google Scholar 

  • Finer J. J. and McMullen M. D. 1990 Transformation of cotton (Gossypium hirsutum L.) via particle bombardment.Plant Cell Rep. 8, 586–589.

    Article  Google Scholar 

  • Gasser C. S. and Fraley R. T. 1989 Genetically engineering plants for crop improvement.Science 244, 1293–1299.

    Article  PubMed  CAS  Google Scholar 

  • Jenkins J. N., McCarty J. C., Buehler R. E., Kiser J., Williams C. and Wofford T. 1997 Resistance of cotton with δ-endotoxin genes fromBacillus thuringiensis var. kurstaki on selected Lepidopteran insects.Agron. J. 89, 768–780.

    Article  Google Scholar 

  • Keller G., Spatola L., McCabe D., Martinell B., Swain W. and John M. E. 1997 Transgenic cotton resistant to herbicide bialaphos.Transgenic Res. 6, 385–392.

    Article  CAS  Google Scholar 

  • Kohel R. J., Quisenberry J. E., Cartwright G. and Yu J. 2000 Linkage analysis of transgenes inserted into cotton via Agrobacterium tumefaciens transformation.J. Cotton Sci. 4, 65–69.

    CAS  Google Scholar 

  • Li Y. E., Chen Z. X., Wu X., Li S. J., Jiao G. L., Wu J. H., Fan X. P., Meng J. H., Zhu Z., Wang W., Zhu Y., Xu H. L., Xiao G. F. and Li X. H. 1998a Obtaining transgenic cotton plants with cowpea trypsin inhibitor gene.Acta Gossypii Sinica 10, 237–243.

    Google Scholar 

  • Li Y. E., Jiao G. L., Li S. J., Fan X. P., Wu X. and Chen Z. X. 1998b Obtaining of homozygous transgenic cotton lines and their genetic analysis.Sci. Agric. Sinica 31, 96–98.

    Google Scholar 

  • Lyon B. R., Cousins Y. L., Llewellyn D. J. and Dennis E. S. 1993 Cotton plants transformed with a bacterial degradation gene are protected from accidental spray drift damage by the herbicide 2,4-dichlorophenoxyacetic acid.Transgenic Res. 2, 162–169.

    Article  CAS  Google Scholar 

  • McCabe D. E. and Martinell B. J. 1993 Transformation of elite cotton cultivars via particle bombardment of meristems.Bio/Technology 11, 596–598.

    Article  Google Scholar 

  • Ni W. C., Zhang Z. L. and Guo S. D. 1998 Development of transgenic insect-resistant cotton plants.Sci. Agric. Sinica 31, 8–13.

    CAS  Google Scholar 

  • Perlak F. J., Deaton R. W., Armstrong R. L., Fuchs R. L., Sims S. R., Greenplate J. T. and Fischhoff D. A. 1990 Insect resistant cotton plants.Bio/Technology 8, 939–943.

    Article  PubMed  CAS  Google Scholar 

  • Rajasekaran K., Grula J. W. and Hudspeth R. L. 1996 Herbicide-resistant Acale and Coker cottons transformation with a native gene encoding mutant forms of acetohydroxyacid synthase.Mol. Breeding 2, 307–319.

    Article  CAS  Google Scholar 

  • Sachs E. S., Benedict J. H., Stelly D. M., Taylor J. F., Altman D. W., Berberich S. A. and Davis S. K. 1998 Expression and segregation of genes encoding CryIA insecticidal proteins in cotton.Crop Sci. 38, 1–11.

    Article  CAS  Google Scholar 

  • Thomas J. C., Adams D. G., Keppenne V. D., Wasmann C. C., Brown J. K., Kanost M. R. and Bohnert H. J. 1995 Protease inhibitors ofManduca sexta expressed in transgenic cotton.Plant Cell Rep. 14, 758–762.

    Article  CAS  Google Scholar 

  • Umbeck P., Johnson G. and Barton K. 1987 Genetically transformed cotton (Gossypium hirsutum L.) plants.Bio/Technology 5, 236–266.

    Article  Google Scholar 

  • Umbeck P., Swain W. and Yang N. S. 1989 Inheritance and expression of genes for kanamycin and chloramphenicol resistance in transgenic cotton plant.Crop Sci. 29, 196–201.

    Article  CAS  Google Scholar 

  • Wang W., Zhu Z. and Deng C. Y. 1998 Obtaining of pest resistant cotton by transforming mediated Agrobacterium. InNew frontiers in cotton research, World Cotton Research Conference-2, p. 119. Athens, Greece.

  • Xie D. X., Fan Y. L., Ni W. C. and Huang J. Q. 1991 TransformedBacillus thuringiensis crystal protein gene into cotton plants.China Sci. B4, 367–373.

    Google Scholar 

  • Zhang B.-H. and Feng R. 1998 Achievement, problems and strategies of transgenic insect-resistant cotton.Acta Agron. Sinica 24, 246–248.

    Google Scholar 

  • Zhang B.-H. and Zhang L. Z. 1998Pest-resistant cotton and its cultivation. Shangdong Science and Technology Press, Jinan.

    Google Scholar 

  • Zhang B.-H. and Zhao B. S. 1997Cotton biotechnology and its application. Chinese Agricultural Press, Beijing.

    Google Scholar 

  • Zhang B.-H., Liu F., Yao C. B. and Wang K. B. 2000 Recent progress in cotton biotechnology and genetic engineering in China.Curr. Sci. 79, 37–44.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Key Laboratory of Cotton Genetic Improvement of the Ministry of Agriculture, Cotton Research Institute, Chinese Academy of Agricultural Sciences, 455112, Anyang Henan, People’s Republic of China

    Bao-Hong Zhang

  2. Institute of Heze Agricultural Sciences, Heze, 273000, Shandong, People’s Republic of China

    Teng-Long Guo

  3. Henan Vocational Technical Teacher’s College, 453003, Xinxiang Henan, People’s Republic of China

    Qing-Lian Wang

Authors
  1. Bao-Hong Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Teng-Long Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qing-Lian Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bao-Hong Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, BH., Guo, TL. & Wang, QL. Inheritance and segregation of exogenous genes in transgenic cotton. J Genet 79, 71–75 (2000). https://doi.org/10.1007/BF02728948

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02728948

Keywords

Search

Navigation