Abstract
Using RNA extracted fromZingiber officinale rhizomes and primers designed according to the conservative regions of monocot mannose-binding lectins, the full-length cDNA ofZ. officinale agglutinin (ZOA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA ofzoa was 746 bp and contained a 510 bp open reading frame (ORF) encoding a lectin precursor of 169 amino acids with a signal peptide. ZOA was a mannose-binding lectin with three typical mannose-binding sites (QDNY). Semi-quantitative RT-PCR analysis revealed thatzoa expressed in all the tested tissues ofZ. officinale including leaf, root and rhizome, suggesting it to be a constitutively expressing form. ZOA protein was successfully expressed inEscherichia coli with the molecular weight expected. To our knowledge, this is the first mannose-binding lectin cDNA cloned from the family Zingiberaceae. Our results demonstrate that monocot mannose-binding lectins also occur within the family Zingiberaceae
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- AAA:
-
Allium ascalonicum agglutinin
- ACPA:
-
Ananas comosus (pineapple) agglutinin
- AKA:
-
Amorphophallus konjac agglutinin
- APA:
-
Allium porrum agglutinin
- ASA:
-
Allium sativum agglutinin
- AUA:
-
Allium ursinum agglutinin
- CAA:
-
Crinum asiaticum agglutinin
- CHA:
-
Cymbidium hybrid agglutinin
- EHA:
-
Epipactis helleborine agglutinin
- GEAFP:
-
Gastrodia elata antifungal protein
- GNA:
-
Galanthus nivalis agglutinin
- LOA:
-
Listera ovata agglutinin
- NHA:
-
Narcissus hybrid agglutinin
- ORF:
-
open reading frame
- PCR:
-
polymerase chain reaction
- RACE:
-
rapid amplification of cDNA ends
- RT-PCR:
-
reverse transcriptase-polymerase chain rection
- TDA:
-
Typhonium divaricatum agglutinin
- TMA:
-
Taxus media agglutinin
- UTR:
-
untranslated region
- ZGA:
-
Zephyrathes grandiflora agglutinin
- ZOA:
-
Zingiber officinale agglutinin
References
Barre A, Bourne Y, Van Damme E J M, Peumans W J and Rouge P 2001 Mannose-binding plant lectins: different structural scaffolds for a common sugar-recognition process;Biochimie 83 645–651
Fei J, Liao Z H, Chai Y R, Pang Y Z, Yao J H, Sun X F and Tang K X 2003 Molecular cloning and characterization of a novel mannose-binding lectin gene fromAmorphophallus konjac;Mol. Biol. Rep. 30 177–183
Haselbeck A, Schickaneder E, Vondereltz H and Hosel W 1990 Structural characterization of glycoprotein carbohydrate chains by using digoxigenin-labeled lectins on blots;Anal. Biochem. 191 25–30
Hilder V A, Powell K S, Gatehouse A M R and Gatehouse J A 1995 Expression of snowdrop lectin in transgenic tobacco plants results in added protection against aphids;Transgenic Res. 4 18–425
Kai G Y, Zheng J G, Zhang L, Pang Y Z, Liao Z H, Li Z G, Zhao L X, Sun X F and Tang K X 2003 Molecular cloning of a new lectin gene fromZephyrathes grandiflora;DNA Seq. 14 335–338
Kai G Y, Zhao L X, Zheng J G, Zhang L, Miao Z Q, Sun X F and Tang K X 2004 Molecular cloning and characterization of a new mannose-binding lectin gene fromTaxus media;J. Biosci. 29 399–407
Kazok M 1981 An analysis of 5′-noncoding sequence from 699 vertebrate messenger RNAs;Nucleic Acids Res. 9 5233–5252
Laemmli U K 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4;Nature (London) 227 680
Peumans W J and Van Damme E J M 1995 Lectins as plant defense proteins;Plant Physiol. 109 347–352
Powell K S, Gatehouse AMR, Hilder V A and Gatehouse J A 1993 Antimetabolic effects of plant-lectins and plant and fungal enzymes on the nymphal stages of 2 important rice pests,Nilaparvata lugens andNephotettix cinciteps;Entomol. Exp. Appl. 66 119–126
Sambrook J, Fritsch E F and Maniatis T 1989Molecular Cloning: A Laboratory Manual, 2nd edition (New York: Cold Spring Harbor Laboratory Press)
Wang X C, Bauw G, Van Damme E J M, Peumans W J, Chen Z L, Van Montagu M, Angenon G and Dillen W 2001 Gastrodianin-like mannose-binding proteins: a novel class of plant proteins with antifungal properties;Plant J. 25 651–661
Van Damme E J M, Allen A K and Peumans W J 1987 Isolation and characterization of a lectin with exclusive specificity towards mannose from snowdrop(Galanthus nivalis) bulbs;FEBS Lett. 215 140–144
Van Damme E J M, Declercq N, Claessens F, Hemschoote K, Peeters B and Peumans W J 1991 Molecular-cloning and characterization of multiple isoforms of the snowdrop(Galanthus-nivalis L.) lectin;Planta 186 35–43
Van Damme E J M, Smeets K, Engelborghs I, Aelbers H, Balzarini J, Pusztai A, Van Leuven F, Goldstein I J and Peumans W J 1993 Cloning and characterization of the lectin cDNA clones from onion, shallot and leek;Plant Mol. Biol. 23 365–376
Van Damme E J M, Smeets K, Torrekens S, Van Leuven F and Peumans W J 1994 Characterization and molecular cloning of mannose-binding lectins from theOrchidaceae speciesListera ovata, Epipactis helleborine andCymbidium hybrid;Eur. J. Biochem. 221 769–777
Van Damme E J M, Peumans W J, Barre B and Rouge P 1998 Plant lectins: a composite of several distinct families of structurally and evolutionary related proteins with diverse biological roles;Crit. Rev. Plant Sci. 17 1–112
Van Damme E J M, Astoul C H, Barre A, Rougé P and Peumans W J 2000 Cloning and characterization of a monocot mannose-binding lectin fromCrocus vernus (family Iridaceae);Eur. J. Biochem. 267 5067–5077
von Heijne G 1986 A new method for predicting signal sequence cleavage sites;Nucleic Acids Res. 14 4683–4690
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chen, Z., Kai, G., Liu, X. et al. cDNA cloning and characterization of a mannose-binding lectin fromZingiber officinaleRoscoe (ginger) rhizomes. J. Biosci. 30, 213–220 (2005). https://doi.org/10.1007/BF02703701
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02703701