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Heat shock but not benzamide and colchicine response elements are present within the — 844 bp upstream region of thehrsω gene ofDrosophila melanogaster

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Abstract

The selective inducibility ofhsrω gene by heat shock and several chemical agents and its selective non-inducibility by heat shock under certain conditions led to suggestion that this locus is subject to multiple controls at the level of transcription. With a view to delimit these different control elements, transgenic lines horbouringhsrω 5’ promoter deletion variants tagged to thelacZ reporter gene were used. Three different assays, viz., staining forβ-galactosidase activity in different larval tissues using chromogenic X-gal substrate, [3H] uridine labelling of polytene nuclei andin situ DNA-DNA hybridization with a non-radioactive probe to polytene chrmosome spreads for checking the puffing status of the resident and the transgene in larval salivary glands, were applied to monitor the activiy of the reporter gene following different treatments. Our results showed that the − 844 bp to +107 bp sequence was sufficient for heat shock induction of the transgene in all tissues. An analysis of the base sequence of thehsrω promoter revealed the presence of three consensus heat shock elements at − 466, − 250 and at − 57 bp and of two GAGA factor binding sites at − 496 and at − 68bp within the − 844 bp region. Germline transformants carrying the − 346 bp to − 844 bp region of thehsrω promoter showed only a very weak heat shock inducibility of the reporter gene in agreement with the presence of only one of the three putative heat shock elements and one of the two GAGA factor binding sites in this region. Interestingly, neither of the transformed lines (carrying the − 844 bp to + 107 bp or the − 844 bp to −346 bp of thehsrω promoter region) showed any response of the transgene to benzamide or colchicine treatments. These results showed that while the heat shock response elements of thehsrω are included within the − 844 bp region the response elements for benzamide and colchicine treatments are outside this region.

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  1. Mousumi Mutsuddi

    Present address: Molecular Biophysics Unit, Indian Institute of Science, 560012, Bangalore, India

Authors and Affiliations

  1. Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, 221 005, Varanasi, India

    S. C. Lakhotia & Mousumi Mutsuddi

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  1. S. C. Lakhotia
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  2. Mousumi Mutsuddi
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Lakhotia, S.C., Mutsuddi, M. Heat shock but not benzamide and colchicine response elements are present within the — 844 bp upstream region of thehrsω gene ofDrosophila melanogaster . J Biosci 21, 235–246 (1996). https://doi.org/10.1007/BF02703111

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  • DOI: https://doi.org/10.1007/BF02703111

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