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Monoclonal antibodies againstWuchereria bancrofti microfilarial excretory-secretory antigens

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Abstract

A battery of monoclonal antibodies were produced againstWuchereria bancrofti microfilarial excretory-secretory antigens and their specificity was studied using different filarial antigens. Among the 1116 wells plated out, 42 % of the wells developed hybrids and 5 % of the hybrids showed antiWuchereria bancrofti microfilarial excretory-secretory antigens. Specificity studies on the antibodies produced from 63 cloned and expanded hybrids showed 10 clones which were specifically positive only toWuchereria bancrofti microfilarial excretory-secretory antigens.

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Abbreviations

Wb mf ES Ag:

Wuchereria bancrofti microfilarial excretory-secretory antigens

DMEM:

Dulbecco’s Modified Eagles Medium

i.p.:

intraperitonially

ELISA:

enzyme linked immunosorbent assay

BSA:

bovine serum albumin

DEA:

diethonolamine

Di Ag:

Dirofilaria immitis Ag

Bm mf Ag:

Brugia malayi mf Ag

Al Ag:

Ascaris lumbricoides Ag

Sd 2–4 Ag:

Setaria digitata 2–4 Ag

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Authors and Affiliations

  1. Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, 442 102, Sevagram, Maharashtra, India

    M. V. R. Reddy, W. F. Piessens & B. C. Harinath

  2. Department of Tropical Public Health, Harvard School of Public Health, 665, Huntington Avenue, 02115, Boston, Massachusetts, USA

    W. F. Piessens

Authors
  1. M. V. R. Reddy
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  2. W. F. Piessens
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  3. B. C. Harinath
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Reddy, M.V.R., Piessens, W.F. & Harinath, B.C. Monoclonal antibodies againstWuchereria bancrofti microfilarial excretory-secretory antigens. J. Biosci. 6, 717–722 (1984). https://doi.org/10.1007/BF02702714

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  • DOI: https://doi.org/10.1007/BF02702714

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