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Effect of stress on the behavior and 5-HT system in Sprague-Dawley and Wistar Kyoto rat strains

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Abstract

The effects of chronic novel stressors, for 21 days, on the behavior and the serotoninergic (5-HT) system in Sprague-Dawley (SD) and Wistar Kyoto (WKY) rats were studied. Open-field and forced-swim tests revealed a significantly greater behavioral depression in the WKY strain. SD rats showed a decrease in3H-DPAT binding to 5-HT1A receptors in the hippocampus, whereas WKY rats revealed an increase in3H-DPAT binding in the hippocampus and hypothalamus. Stress did not appear to alter the binding of3H-DPAT to 5-HT1A sites in the dorsal raphe or median raphe in either strains. SD rats revealed a modest increase in 5-HT transporter (5-HTT) sites in the cortex; WKY rats revealed a decrease in 5-HTT sites in the cortex and the hippocampus. Stress caused an increase in3H-CNIMI binding to 5-HTT sites in the dorsal and median raphe nuclei in both strains. The results suggest that the greater susceptibility to behavioral depression in WKY rats may account for the differential effects on 5HT1A sites as well as 5-HTT sites in limbic regions and cell body area as compared to SD rats.

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References

  • Adell, A., R. Trullas & E. Gelpi (1988). Time course of changes in serotonin and noradrenaline in rat brain after predictable or unpredictable shock.Brain Research 459 54–59.

    Article  PubMed  Google Scholar 

  • Anisman, H., & R. Zacharko (1982). Depression: The predisposing influence of stress.Behavioral Brain Research 5 89–137.

    Article  Google Scholar 

  • Chaouloff, F. (1994). Effects of repeated 2-Deoxy-D-glucose administration on ingestive, psychological and 5-HT-related behaviors in the rat.Neuropharmacology 13 693–703.

    Article  Google Scholar 

  • Clement, H.W., F. Schafer, C. Ruwe, D. Gemsa & W. Wesemann (1993). Stress-induced changes in extracellular 5-hydroxyindoleacetic acid concentrations followed in the nucleus raphe dorsalis and the frontal cortex of the rat.Brain Research 614 117–124.

    Article  PubMed  Google Scholar 

  • Edwards, E., W. Komrich, P. V. Houtten & F.A. Henn (1992). Presynaptic serotonin mechanisms in rats subjected to inescapable shock.Neuropharmacology 31 323–330.

    Article  PubMed  Google Scholar 

  • Hamon, M., H. Gozlan, S. E. Mestikawy, M.B. Emerit, F. Bolanos & L. Schecter (1990). Annals of the New York Academy of Science,600: 114–131.

    Article  Google Scholar 

  • Hensler, J.G., G. B. Kovachich & A. Frazer (1991). A quantitative autoradiographic study of serotonin1A receptors regulation. Effect of 5, 7-dihydroxytryptamine and antidepressant treatments.Neuropsychopharmacology 4 131–143.

    PubMed  Google Scholar 

  • Ikeda, M., Y. Hirata, K. Fujita, M. Shimzato, H. Takahashi, S. Yagyu & T. Nagatsu (1984). Effects of stress on release of dopamine and serotonin in the striatum of spontaneously hypertensive rats.Neurochemistry International 6 509–512.

    Article  PubMed  Google Scholar 

  • Jaffe, E.H., V. Defrias & C. Ibarrah (1993). Changes on basal and stimulated release of endogenous serotonin from different nuclei of rats subjected to two models of depression.Neuroscience Letters 162 157–160.

    Article  PubMed  Google Scholar 

  • Katz, R.J., K.A. Rotha & B.J. Carroll (1981). Animal models and human depressive disorders.Neuroscience Biobehavioral Reviews 5 247–251.

    Article  Google Scholar 

  • Kennett, G.A., D. L. Dickinson & G. Curzon (1985). Enhancement of some 5-HT dependent behavioral responses following repeated immobilization in rats.Brain Research 330 253–263.

    Article  PubMed  Google Scholar 

  • Korte, S.M., S. Van Duin, G.A.H. Bouws, J.M. Koolhaus & B. Bohus (1991). Involvement of hypothalamic serotonin in activation of the sympathoadrenomedullary system and hypothalamo-pituitary-adrenocortical axis in male Wistar rats.European Journal Pharmacology 197 225–228.

    Article  Google Scholar 

  • Kosofsky, B.E. & M. E. Molliver (1987). The serotoninergic innervation of cerebral cortex: Different classes of axon terminals arise from dorsal and median raphe nuclei.Synapse 1 153–168.

    Article  PubMed  Google Scholar 

  • Kovachich, G.B., C.E. Aronson & D. J. Brunswick (1988). Quantitative autoradiography of serotonin uptake sites in rat brain using [3H]cyanoimipramine.Brain Research 454 78–88.

    Article  PubMed  Google Scholar 

  • Lucki, I. (1992). 5-HT-1 receptors and behavior.Neuroscience & Biobehavioral Reviews, 16 83–93.

    Article  Google Scholar 

  • Malyszki, J., Y. Takada, T. Urano, K. Serizawa, Y. Kozima, D. Yan & A. Takada (1994). Peripheral and central serotonergic systems in rats subjected to footshock or water-immersion restraint stress.Biogenic Amines 10 303–310.

    Google Scholar 

  • Malyszki, J., T. Urano, Y. Takada, & A. Takada (1995). Amino acids, serotonin and 5-hydroxyindole acetic acid following foot shock in rats.Brain Research Bulletin 36 137–140.

    Article  Google Scholar 

  • Mendelson, S.D. & B. S. McEwen (1992). Autoradiographic analyses of the effects of adrenalectomy and corticosterone on 5-HT1A and 5-HT1B receptors in the dorsal hippocampus and cortex of the rat.Neuroendocrinology 55 444–450.

    Article  PubMed  Google Scholar 

  • Nankai, M., S. Yamada, & M. Turo (1991). Down regulation of serotonin uptake sites in rat brain induced by concomitant chronic administration of desipramine and repeated stress.Japanese Journal of Psychiatry 45, 105–106.

    Google Scholar 

  • Okamoto, K. & K. Aoki (1963). Development of a strain of spontaneously hypertensive rats.Japanese Circulation Journal 27 282–293.

    Article  PubMed  Google Scholar 

  • Paré, W.P. (1989). Stress ulcer susceptibility and depression in Wistar Kyoto (WKY) rats.Physiology & Behavior 46 993–998.

    Article  Google Scholar 

  • Paré, W.P. & E. Redei (1993). Depressive behavior and stress ulcer in Wistar Kyoto rats.Journal Physiology (Paris)87 229–238.

    Google Scholar 

  • Paxinos, G. & C. Watson (1986).The rat brain in stereotaxic coordinates. NY: Academic Press.

    Google Scholar 

  • Petty, F., G. Kramer & L. Wilson (1992). Prevention of learned helplessness: In vivo correlation with cortical serotonin,Pharmacology Biochemistry Behavioral 48 361–367.

    Article  Google Scholar 

  • Redei, E., W. Paré, F. Aird & J. Kluczynski (1994). Stress differences in hypothalamic-pituitary-adrenal activity and stress ulcer.American Journal Physiology 266 R353-R360.

    Google Scholar 

  • SAS/STAT User’s Guide. Version 6, 4th ed., Cary, N.C.: SAS Institute Inc. (1989).

  • Tejani-Butt, S.M., W. P. Paré & J. Yang (1994). Effect of repeated novel stressors on depressive behavior and brain norepinephrine receptor system in Sprague-Dawley and Wistar Kyoto (WKY) rats.Brain Research 649 27–35.

    Article  PubMed  Google Scholar 

  • Tejani-Butt, S.M. & D. M. Labow (1994). Time course of the effects of adrenalectomy and corticosterone replacement on 5-HT1A receptors and 5-HT uptake sites in the hippocampus and dorsal raphe nucleus of the rat brain: An autoradiographic analysis.Psychopharmacology, 113 481–486.

    Article  PubMed  Google Scholar 

  • Watanabe, Y., R. R. Sakai, B. S. McEwen & S. Mendelson (1993). Stress and antidepressant effects on hippocampal and cortical 5-HT1A and 5-HT2 receptors and transport sites for serotonin.Brain Research 615 87–94.

    Article  PubMed  Google Scholar 

  • Weiss, J.M., P.A. Goodman, B.A. Losito, S. Corrigan, J.M. Charney & W.H. Bailey (1981). Behavioral depression produced by an uncontrollable stressor: Relationship to norepinephrine, dopamine and serotonin levels in various regions of rat brain.Brain Research Reviews 3 167–205.

    Article  Google Scholar 

  • Willner, P. (1985). Antidepressants and serotonergic neurotransmission: An integrative review.Psychopharmacology 85: 387–404.

    Article  PubMed  Google Scholar 

  • Willner, P. (1990). Animal models of depression, an overview.Pharmacology & Therapeutics 45 425–455.

    Article  Google Scholar 

  • Yavari, P., G. W. Vogel & D. B. Neill (1993). Decreased raphe unit activity in a rat model of endogenous depression.Brain Research 611 31–36.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Pavlovian Research Laboratory, VA Medical Center, 21902, Perry Point, MD

    William P. Paré (Chief)

  2. Department of Psychiatry, Univ. of Pennsylvania, 19104, Philadelphia, PA

    Shanaz M. Tejani-Butt (Research Assistant Professor)

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  1. William P. Paré
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  2. Shanaz M. Tejani-Butt
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Paré, W.P., Tejani-Butt, S.M. Effect of stress on the behavior and 5-HT system in Sprague-Dawley and Wistar Kyoto rat strains. Integrative Physiological and Behavioral Science 31, 112–121 (1996). https://doi.org/10.1007/BF02699783

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