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Analysis for geometrical and positional isomers of fatty acids in partially hydrogenated fats

  • Published:
Journal of the American Oil Chemist’s Society

Abstract

A liquid Chromatographic procedure for frac-tionation and analysis of methyl esters from fats is described. First, esters are separated by liquid chromatography on a partially vulcanized rubber column into trienoate, dienoate, monoenoate-palmitate and stearate fractions. The monoenoate-palmitate fraction is separated by liquid chro-matography on a silver-saturated cation exchange resin into palmitate,trans monoenoate and cis monoenoate fractions. Double bond positions intrans and cis monoenoates are located by ozoniza-tion and gas chromatography of fragments. This separation procedure requires less time and uses simpler apparatus with smaller samples than a previously described method based on counter-current distribution. Data from analyses of two liquid oils and four shortenings are presented.

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Authors and Affiliations

  1. Northern Regional Research Laboratory, Peoria, Illinois

    C. R. Scholfield, V. L. Da Vison & H. J. Dutton

Authors
  1. C. R. Scholfield
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  2. V. L. Da Vison
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  3. H. J. Dutton
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Additional information

No. Utiliz. Ees. Dev. Div., ARS, USDA.

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Scholfield, C.R., Vison, V.L.D. & Dutton, H.J. Analysis for geometrical and positional isomers of fatty acids in partially hydrogenated fats. J Am Oil Chem Soc 44, 648–651 (1967). https://doi.org/10.1007/BF02680035

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  • DOI: https://doi.org/10.1007/BF02680035

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