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Behavior of diglycerides and conjugated fatty acid triglycerides in reverse-phase chromatography

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  • Published:
Journal of the American Oil Chemists’ Society

Abstract

The behavior of conjugated fatty acid triglycerides and diglycerides on reverse-phase chromatography was studied. Trieleostearin is a geometric isomer of trilinolenin. The conjugated double bond arrangement in trieleostearin enhances its hydrophobic interaction with the stationary phase and causes it to be eluted later than trilinolenin. In separation of “critical pairs” of tri- and diglycerides, diglycerides elute later than triglycerides due to the longer fatty acid constituent. Position isomers of 1,2- and 1,3-diglycerides can be separated by reverse-phase high-performance liquid chromatography.

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Authors and Affiliations

  1. SRRC, ARS, USDA, P.O. Box 19687, 70179, New Orleans, Louisiana

    M. K. Chang, E. J. Conkerton, D. Chapital & P. J. Wan

Authors
  1. M. K. Chang
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  2. E. J. Conkerton
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  3. D. Chapital
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  4. P. J. Wan
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Chang, M.K., Conkerton, E.J., Chapital, D. et al. Behavior of diglycerides and conjugated fatty acid triglycerides in reverse-phase chromatography. J Am Oil Chem Soc 71, 1173–1175 (1994). https://doi.org/10.1007/BF02675915

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  • DOI: https://doi.org/10.1007/BF02675915

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