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Separation of tetra-, penta-, and hexaacyl triglycerides by high performance liquid chromatography

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Lipids

Abstract

Synthetic triglycerides with more than three acyl groups were prepared by forming estolides from triglycerides containing one, two, or three monohydroxy fatty acyl moieties. These tetra-, penta-, and hexaacyl triglycerides were examined by high performance liquid chromatography (HPLC) and thin layer chromatography (TLC). HPLC separation by the number of acyl groups was obtained with both conventional phase and reverse phase columns. In all systems, triglycerides were eluted first, followed in sequence by tetra-, penta-, and hexaacyl triglycerides. Within each glyceride class, further separation occurred due to variations in chain length and degree of unsaturation among the component acids. TLC migration on silica gel was found to be a function of the number of acyl groups and the length of component acid chains.

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Authors and Affiliations

  1. Northern Regional Research Center, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, 61604, Peoria, IL

    Kathleen Payne-Wahl, Ronald D. Plattner, Gayland F. Spencer & Robert Kleiman

Authors
  1. Kathleen Payne-Wahl
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  2. Ronald D. Plattner
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  3. Gayland F. Spencer
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  4. Robert Kleiman
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The mention of firm names or trade products does not imply that they are endorsed or recommended by the U.S. Department of Agriculture over other firms or similar products not mentioned.

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Payne-Wahl, K., Plattner, R.D., Spencer, G.F. et al. Separation of tetra-, penta-, and hexaacyl triglycerides by high performance liquid chromatography. Lipids 14, 601–605 (1979). https://doi.org/10.1007/BF02533443

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  • DOI: https://doi.org/10.1007/BF02533443

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