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Preparation of mitochondrial DNAs from sunflowers (Helianthus annuus L.) and from beets (Beta vulgaris) using a medium with a high ionic strength

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Abstract

The isolation of mitochondria from sugar beet and sunflower has been carried outina buffered medium of high ionic strength (I=1.40 M). Them tDNAs obtained were enriched for supercoiled molecules of low molecular weight. Chloroplast and mitochondrial DNA were successively prepared and used from a single leaf preparation for further analysis of restriction fragment length polymorphism. The few available wild relatives of sugar beet or sunflower have also been analyzed by comparison of the restriction patterns of their chloroplast and mitochondrial DNAs.

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Abbreviations

CCC:

covalently closed circular

CMS:

cytoplasmic male sterile

DEPC:

diethyl pyrocarbonate

MF:

male-fertile

MS:

male-sterile

OC:

open cricle

pO:

minicirclec of sugar beet in mitochondria

pIT:

mitochondrial plasmid of sunflower

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Authors and Affiliations

  1. Station de Génétique et d'Amélioration des Plantes, Laboratoire de Génétique et Mutagenèse, Institut National de la Recherche Agronomique, BV 1540, 21034, Dijon Cédex, France

    Christine Pérez, Jean-François Bonavent & André Bervillé

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  1. Christine Pérez
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  2. Jean-François Bonavent
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  3. André Bervillé
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Pérez, C., Bonavent, JF. & Bervillé, A. Preparation of mitochondrial DNAs from sunflowers (Helianthus annuus L.) and from beets (Beta vulgaris) using a medium with a high ionic strength. Plant Mol Biol Rep 8, 104–113 (1990). https://doi.org/10.1007/BF02669764

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