Abstract
The techniques of microdissection and microcloning have been applied to the isolation of B-chromosome DNA from rye. We have identified a DNA sequence on the rye B-chromosome which is homologous to an A-chromosome sequence, and which is dispersed and moderately repeated on the A- and B-chromosomes. This demonstrates that the rye B-chromosome is heterogeneous in the nature of its DNA sequence composition, containing sequences which are present on the A-chromosomes in addition to those not present on the A-chromosomes.
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Abbreviations
- UV:
-
ultraviolet light
- PEG:
-
polyethylene glycol
- DEAE:
-
diethylaminoethyl
- PCR:
-
polymerase chain reaction
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Sandery, M.J., Forster, J.W., Macadam, S.R. et al. Isolation of a sequence common to A- and B-chromosomes of rye (Secale cereale) by microcloning. Plant Mol Biol Rep 9, 21–30 (1991). https://doi.org/10.1007/BF02669286
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DOI: https://doi.org/10.1007/BF02669286