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The heterogeneity of B-chromosome DNA: no evidence for a B-chromosome specific repetitive DNA correlated with B-chromosome effects on meiotic pairing in the triticinae

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Abstract

The compositional heterogeneity of DNAs of A (normal) and B (supernumerary) chromosomes of Aegilops speltoides, Ae. mutica and Triticum aestivum has been compared in order to elucidate the mechanism of B-chromosome disruption of meiotic pairing in interspecific hybrids. Comparisons of % heterologous association after DNA/DNA hybridation at C0t 10−2 (highly repetitious DNA) and C0t 100 (moderately repetitious DNA), and comparisons of nucleotide base divergence (ΔTms) and thermal elution profiles of homologous and heterologous duplexes, show that genotypes of Aegilops spp., having large numbers of Bs, do not carry additional families of repetitious DNA exclusive to B-chromosomes. Neither the presence of Bs nor the direction of DNA/DNA hybridisation affect the above parameters. No cryptic DNA satellites were revealed in A- and B-chromosome DNA after sedimentation in actinomycin D-CsCl gradients; and there were no significant differences in buoyant densities of main-band DNA. Mean melting temperatures (Tm); transition temperatures (ΔT) and numbers and positions of peaks of dissociating DNA fractions in profiles of differentiated melting curves of native DNAs were similar in strictly comparable denaturation conditions. One small AT-rich (< 5%) DNA fraction correlated with speltoides Bs was revealed; however, no corresponding fraction is associated with mutica Bs. The overall similarity in numbers and base composition of families of DNA (repetitious and unique) of As and Bs is discussed in relation to the origin of Bs and the origin of the meiotic diploidising system in haploid T. aestivum.

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  1. Department of Genetics, University of Cambridge, Milton Road, CB4 1XH, Cambridge, England

    Gabriel A. Dover

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  1. Gabriel A. Dover
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Dover, G.A. The heterogeneity of B-chromosome DNA: no evidence for a B-chromosome specific repetitive DNA correlated with B-chromosome effects on meiotic pairing in the triticinae. Chromosoma 53, 153–173 (1975). https://doi.org/10.1007/BF00333043

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  • DOI: https://doi.org/10.1007/BF00333043

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