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Construction and characterization of a bacterial artificial chromosome library ofArabidopsis thaliana

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Abstract

We constructed an ordered 3,948-clone arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library.

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Abbreviations

BAC:

bacterial artificial chromosome

IPTG:

isopropylthio-β-D-galactoside

LB:

Luria-Bertani medium

YAC:

yeast artificial chromosome

X-Gal:

5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside

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Authors and Affiliations

  1. Crop Biotechnology Center Texas A&M University, 77843, College Station, TX, USA

    Sangdun Choi, Robert A. Creelman, John E. Mullet & Rod A. Wing

Authors
  1. Sangdun Choi
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  2. Robert A. Creelman
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  3. John E. Mullet
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  4. Rod A. Wing
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Corresponding author

Correspondence to Rod A. Wing.

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Choi, S., Creelman, R.A., Mullet, J.E. et al. Construction and characterization of a bacterial artificial chromosome library ofArabidopsis thaliana . Plant Mol Biol Rep 13, 124–128 (1995). https://doi.org/10.1007/BF02668782

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  • DOI: https://doi.org/10.1007/BF02668782

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