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Differential expression of fructosyllysine-specific receptors on monocytes and macrophages and possible pathophysiological significance

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Summary

A differing individual expression of fructosyllysine-specific receptors has been found on the monocytes of 90 insulin-dependent diabetic patients and 101 healthy control subjects. The degree of receptor expression is neither age- nor sex-dependent; however, in the diabetic group it correlates significantly with the severity and age of onset of diabetic microangiopathy. To interpret the results of the human study, spontaneously diabetic and non-diabetic BB/OK rats were used to estimate tissue content of glucose-modified proteins and capillary basement membrane thickness in relation to the receptor expression on macrophages. In non-diabetic and diabetic rats no correlation was found between receptor expression and tissue content (i.e. artery, nerve) of fructosyllysine and fluorescent advanced glycation end products. However, animals which express the fructosyllysine receptor showed a greater increase in muscle capillary basement membrane thickness. There are indications that fructosyllysine receptor expression is positively associated with indices of diabetic complications such as microangiopathy and/or capillary basement membrane thickening.

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Abbreviations

AGE:

Advanced glycation end products

FITC:

fluorescein isothiocynate

FL:

fructosyllysine

GHb:

total glycated haemoglobin

PBS:

phosphate buffered saline

TBS:

Tris buffered saline

CV:

coefficient of variation

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Authors and Affiliations

  1. Institute of Biochemistry, Faculty of Medicine, Ernst Moritz Arndt University, Greifswald, Germany

    R. Brandt, C. Landmesser, K. Hartmann & S. Krantz

  2. Institute of Diabetes, Karlsburg, Faculty of Medicine, Ernst Moritz Arndt University, Greifswald, Germany

    L. Vogt & B. Hehmke

  3. Laboratory of Electron Microscopy, Department of Biology, Ernst Moritz Arndt University, Greifswald, Germany

    R. Hanschke

  4. Laboratory of Electron Microscopy, Department of Geological Sciences, Ernst Moritz Arndt University, Greifswald, Germany

    J. Kasbohm

  5. Institute of Biometry and Medical Informatics, Faculty of Medicine, Ernst Moritz Arndt University, Greifswald, Germany

    B. Jäger

  6. Department of Diabetes, Klinikum Karlsburg, Karlsburg, Germany

    D. Michaelis

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  1. R. Brandt
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  2. C. Landmesser
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  3. L. Vogt
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  4. B. Hehmke
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  5. R. Hanschke
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  6. J. Kasbohm
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  7. K. Hartmann
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  8. B. Jäger
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  9. S. Krantz
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  10. D. Michaelis
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Brandt, R., Landmesser, C., Vogt, L. et al. Differential expression of fructosyllysine-specific receptors on monocytes and macrophages and possible pathophysiological significance. Diabetologia 39, 1140–1147 (1996). https://doi.org/10.1007/BF02658499

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  • DOI: https://doi.org/10.1007/BF02658499

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