Summary
A procedure is described whereby the mixed fatty acids of pork liver lipid are fractionated into groups of acids to facilitate their analysis and isolation. The major acids of pork liver lipid were palmitic 22.9%, stearic 16.8%, oleic 38.5%, linoleic 5.0%, and arachidonic 4.4%. A number of other unsaturated fatty acids were detected in minor amounts, such as isomers of arachidonic, linoleic, and palmitoleic acids. A number of fatty acids of odd-numbered chain length also were detected.
The isolation of methyl arachidonate in a number of preparations of purity ranging from 87 to more than 99% is described. Yields ranged from 44% for the purest arachidonate to 93% for the less pure concentrates. Alkali-isomerization analyses of methyl arachidonate from this source, by the 21% KOH-glycol method, gave appreciably different absorptivities from those previously published.
Various criteria for estimating purity of polyun-saturated fatty acids are discussed in the light of results of the analysis of methyl arachidonate.
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References
Ault, W. C., and Brown, J. B., J. Biol. Chem.,107, 615–622 (1934).
Brown, J. B., J. Biol. Chem.,80, 455–460 (1928).
Dolby, D. E., Nunn, L. C. A., and Smedley-MacLean, I., Biochem. J.,34, 1422–1426 (1940).
Farmer, E. H., and Van den Heuvel, F. A., J. Chem. Soc.,1938, 427–430.
Farmer, E. H., and Van den Heuvel, F. A., J. Soc. Chem. Ind.,57, 24–31T (1938).
Hammond, E. G., and Lundberg, W. O., J. Am. Oil Chemists’ Soc.,30, 438–441 (1953).
Herb, S. F., and Riemenschneider, R. W., and Donaldson, J., J. Am. Oil Chemists’ Soc.,28, 55–58 (1951).
Herb, S. F., and Riemenschneider, R. W., J. Am. Oil Chemists’ Soc.,29, 456–461 (1952).
Herb, S. F., and Riemenschneider, R. W., paper presented at the spring meeting of the American Oil Chemists’ Society, New Orleans, 1959.
Howard, G. A., and Martin, J. P., Biochem J.,46, 532–538 (1950).
Irving, E., and Smith, J. A. B., Biochem J.,29, 1358–1368 (1935).
Kyte, R. M., J. Am. Oil Chemists’ Soc.,33, 146–149 (1956).
Mowry, D. T., Brode, W. R., and Brown, J. B., J. Biol. Chem.,142, 671–678 (1942).
Von Rudloff, E., J. Am. Oil Chemists’ Soc.,33, 126–128 (1956).
Schlenk, H., Gellerman, J. L., Tillotson, J. A., and Mangold, H. K., J. Am. Oil Chemists’ Soc.,34, 377–386 (1957).
Shinowara, G. Y., and Brown, J. B., J. Biol. Chem.,134, 331–340 (1940).
Stoffel, W., and Ahrens, E. H., J. Am. Chem. Soc.,80, 6604–6608 (1958).
Tsujimoto, M., J. Chem. Ind. (Japan),23, 1007–1010 (1920).
White, M. F., and Brown, J. B., J. Am. Chem. Soc.,70, 4269–4270 (1948).
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This work is supported by funds from the Hormel Foundation.
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Privett, O.S., Weber, R.P. & Nickell, E.C. Preparation and properties of methyl arachidonate from pork liver. J Am Oil Chem Soc 36, 443–449 (1959). https://doi.org/10.1007/BF02639637
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DOI: https://doi.org/10.1007/BF02639637