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Effects of organic solvents on transesterification of phospholipids using phospholipase A2 and lipase

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Abstract

The transesterification abilities of phospholipase A2 and Mucor javanicus lipase in various organic solvents were studied. Phosphatidylcholine and caprylic acid (molar ratio, 1:6) were transesterified by incubation at 40°C in three organic solvents using phospholipase A2 and M. javanicus lipase. The fatty acid composition of the reaction product was measured using gas chromatography. Caprylic acid was incorporated into the sn-1 and -2 positions at a rate of 87.7% using phospholipase A2 in hexane, and 36.7% using M. javanicus lipase in diethyl ether. Higher acyl migration into sn-2 was observed in diethyl ether than in hexane during transesterification using M. javanicus lipase, however, there was no substantial difference in the caprylic acid content at the sn-2 position. Acyl migration during transesterification in methanol was lower than in other organic solvents. Non-polar hexane and diethyl ether were more effective for transesterification than methanol.

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Authors and Affiliations

  1. Korea Food Research Institute, Seongnam, Gyeonggi, 463-746, Korea

    Jin Hee Kim

  2. Autel Co., Ltd., Seoul, 138-865, Korea

    Suk Hoo Yoon

Authors
  1. Jin Hee Kim
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  2. Suk Hoo Yoon
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Correspondence to Suk Hoo Yoon.

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Kim, J.H., Yoon, S.H. Effects of organic solvents on transesterification of phospholipids using phospholipase A2 and lipase. Food Sci Biotechnol 23, 1207–1211 (2014). https://doi.org/10.1007/s10068-014-0165-6

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  • DOI: https://doi.org/10.1007/s10068-014-0165-6

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