Skip to main content
SpringerLink
Log in

Characterization of wax esters in the roe oil of amber fish,Seriola aureovittata

  • Published:
Journal of the American Oil Chemists’ Society

Abstract

The wax esters of the roe oil of the amber fish,Seriola aureovittata, have been resolved by high-performance liquid chromatography (HPLC) in the silver-ion mode. Each of the fractions collected was transmethylated, and the fatty acids and alcohols were identified by gas chromatography/mass spectrometry (GC/MS) as the picolinyl esters and nicotinates, respectively. Their compositions were determined by GC. The fatty acid composition is complex, and the main components are C18:1n-9 (35.5 mol%), C22:6n-3 (20.3 mol%), and C16:1n-7 (10.7 mol%), while fatty alcohols are limited to saturated (C16:0, 60.3 mol%; C18:0, 15.3 mol%; C14:0, 5.1 mol%) and monoenoic alcohols (C18:1n-9, 6.5 mol%; C16:1n-7, 4.5 mol%) with traces (<0.1 mol%) of polyunsaturated fatty alcohols such as C20:3n-3, C20:4n-6, C20:5n-3, and C22:5n-3. Silver-ion HPLC exhibited excellent resolution in which fractions were resolved on the basis of the number and configuration of double bonds as well as the distribution pattern between the acid and alcohol moieties of the molecules with a given number of double bonds. The main wax ester fraction are those of monoenoic acid-saturated alcohol species, hexaenoic acid/saturated alcohol species, and pentaenoic acid/saturated alcohol species. Appreciable specificity was observed in the esterification of fatty acids with alcohols, and surprisingly, no saturated acid-monoenoic alcohol species were detected.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Christie, W.W.,High-Performance Liquid Chromatography and Lipids, Pergamon Press, Oxford, 1987, pp. 172–210.

    Google Scholar 

  2. DeRuyter, M.G.M., and A.P. DeLeenheer,Clin. Chem. 24:1920 (1978).

    CAS  Google Scholar 

  3. Baht, P.V., and A. Lacroix,J. Chromatogr. 272:269 (1983).

    Article  Google Scholar 

  4. May, H.E., and S.I. Koo,J. Liquid Chromatogr. 12:1261 (1989).

    CAS  Google Scholar 

  5. Perkins, E.G., D.J. Hendren, J.E. Bauer and A. El-Hamdy,Lipids 16:609 (1981).

    Article  CAS  Google Scholar 

  6. Billheimer, J.T., S. Avart and B. Milani,J. Lipid Res. 24:1646 (1983).

    CAS  Google Scholar 

  7. Spencer, G.R., R.D. Plattner and T. Miwa,J. Am. Oil Chem. Soc. 54:187 (1977).

    CAS  Google Scholar 

  8. Miwa, T.K., Ibid:407 (1984).

    CAS  Google Scholar 

  9. Nevenzel, J.C., W. Rodegker, J.S. Robinson and M. Kayama,Comp. Biochem. Physiol. 31:25 (1969).

    Article  CAS  Google Scholar 

  10. Takagi, T., Y. Itabashi, T. Ota and K. Hayashi,Lipids 11:354 (1976).

    Article  CAS  Google Scholar 

  11. Spencer, G.F.,J. Am. Oil Chem. Soc. 56:642 (1979).

    CAS  Google Scholar 

  12. Plattner, R.D., and G.F. Spencer,Lipids 18:68 (1983).

    Article  CAS  Google Scholar 

  13. Challinor, C.J., R.J. Hamilton and K. Simpson,Chem. Phys. Lipids 3:145 (1969).

    Article  CAS  Google Scholar 

  14. Christie, W.W., E.Y. Brechany and K. Stefanov, Ibid.:127 (1988).

    Article  CAS  Google Scholar 

  15. Stefanov, K., M. Konaklieva, E.Y. Brechany and W.W. Christie,Phytochem. 27:3495 (1988).

    Article  CAS  Google Scholar 

  16. Christie, W.W., E.Y. Brechany and V.K.S. Shukla,Lipids 24:116 (1989).

    Article  CAS  Google Scholar 

  17. Christie, W.W., and G.H.M. Breckenridge,J. Chromatogr. 469:261 (1989).

    Article  CAS  Google Scholar 

  18. Christie, W.W., E.Y. Brechany, K. Stefanov and S. Popov,Lipids 27:640 (1992).

    Article  CAS  Google Scholar 

  19. Christie, W.W.,J. Chromatogr. 454:273 (1988).

    Article  CAS  Google Scholar 

  20. Nikolova-Damyanova, B., W.W. Christie and B. Herslof,J. Am. Oil Chem. Soc. 67:503 (1990).

    Article  CAS  Google Scholar 

  21. Christie, W.W.,Fat Sci. Technol. 93:65 (1991).

    CAS  Google Scholar 

  22. Santinelli, F., P. Damiani and W.W. Christie,J. Am. Oil Chem. Soc. 69:552 (1992).

    CAS  Google Scholar 

  23. Laakso, P., and W.W. Christie,Lipids 25:349 (1990).

    Article  CAS  Google Scholar 

  24. Laakso, P., and W.W. Christie,J. Am. Oil Chem. Soc. 68:213 (1991).

    CAS  Google Scholar 

  25. Choi, E.J., M.Sc. Thesis, Dong-A University, Pusan, Korea, 1991.

  26. Christie, W.W.,J. High Resolut. Chromatogr. Chromatogr. Commun. 10:148 (1987).

    Article  CAS  Google Scholar 

  27. Christie, W.W.,Gas Chromatography and Lipids, Oily Press, Ayr, 1989, pp. 68–69.

    Google Scholar 

  28. Balazy, M., and A.S. Nies,Biomed. Environ. Mass Spectrom. 18:328 (1989).

    Article  CAS  Google Scholar 

  29. Villani, F.J., and M.S. King,Org. Synth. Coll. 4:88 (1963).

    Google Scholar 

  30. Harvey, D.J., inAdvances in Lipid Methodology—One, edited by W.W. Christie, Oily Press, Ayr, 1989, pp. 19–80.

    Google Scholar 

  31. Harvey, D.J.,Biomed. Environ. Mass Spectrom. 18:719 (1989).

    Article  CAS  Google Scholar 

  32. Bertelson, O., and Ng Dinh-Nguyen,Fette Seifen Anstrichm. 87:336 (1985).

    Article  Google Scholar 

  33. Christie, W.W.,Gas Chromatography and Lipids, Oily Press, Ayr, 1989, pp. 110–111.

    Google Scholar 

  34. Iyengar, R., and H. Schlenk,Biochemistry 6:396 (1967).

    Article  CAS  Google Scholar 

  35. Mori, M., and T. Saito,Bull. Japan. Soc. Sci. Fish. 32:730 (1966).

    CAS  Google Scholar 

  36. Mori, M., S. Yasuda and S. Nishimuro, Ibid.:363 (1978).

    CAS  Google Scholar 

  37. Sand, D.M., and H. Schlenk,Lipids 4:303 (1969).

    Article  CAS  Google Scholar 

  38. Nikolova-Damyanova, B., B. Herslof and W.W. Christie,J. Chromatogr. 609:133 (1992).

    Article  CAS  Google Scholar 

  39. Mori, M., T. Saito and Y. Nakanishi,Bull. Jap. Soc. Sci. Fish. 32:668 (1966).

    CAS  Google Scholar 

  40. Sano, Y., Ibid.:734 (1966).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Food Science and Nutrition, Dong-A University, 604-174, Pusan, Korea

    Yong-Goe Joh

  2. The Hannah Research Institute, KA6 5HL, Ayr, Scotland, United Kingdom

    Elizabeth Y. Brechany

  3. The Scottish Crop Research Institute, Invergowrie, DD2 5DA, Dundee, Scotland, United Kingdom

    William W. Christie

Authors
  1. Yong-Goe Joh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Elizabeth Y. Brechany
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. William W. Christie
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Joh, YG., Brechany, E.Y. & Christie, W.W. Characterization of wax esters in the roe oil of amber fish,Seriola aureovittata . J Am Oil Chem Soc 72, 707–713 (1995). https://doi.org/10.1007/BF02635660

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02635660

Key words

Search

Navigation